Objective:To analyze the pathogen carrying status and pathogenic causes of porcine Escherichia coli after PCR detection.Methods:From January to December 2022,97 strains of Escherichia coli were isolated from pig farms,40 strains were isolated from the feces of pregnant sows,and 24 strains were isolated from the feces of weaned piglets.The virulence gene carrying status and pathogenic causes of Escherichia coli in different populations were analyzed.Results:Group A accounted for 42.27%,group B1 accounted for 56.70%,group B2 accounted for 1.03%,group D accounted for 6.19%,group B1 was the dominant group.Among 40 strains of E.coli in pregnant sows,8 strains and 2 strains were detected by EAEC and EHEC.A total of 10 strains were detected.Among 33 strains of E.coli in lactating sows,15 strains were detected by EAEC.Among the 24 strains of Escherichia coli of weaned piglets,8 strains were detected by EAEC,1 strain by ETEC,4 strains by EPEC and 1 strain by EEC-EPEC.A total of 39 strains.Conclusion:Serious pollution of the environment around the pig farm is caused by the excretion of porcine Escherichia coli from the manure.In addition,incorrect storage methods and transportation stages during the slaughter of pigs can increase the risk of porcine Escherichia coli infection.