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猪大肠埃希菌的PCR检测结果分析

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目的:分析猪大肠埃希菌采用PCR检测后,其菌株病菌携带情况以及致病原因.方法:于2022年1-12月,从养猪场分离出97株大肠埃希菌,40株从妊娠母猪粪便中分离出来,24株从断奶仔猪粪便中分离出来.分析其不同种群大肠埃希菌的毒力基因携带情况以及致病原因.结果:A组占42.27%、B1组占56.70%、B2组占1.03%、D组占6.19%,B1组为优势组.在40株妊娠母猪大肠杆菌中,EAEC检出8株、EHEC检出2株,共检出10株;在33株哺乳母猪大肠杆菌中,EAEC检出15株;在24株断奶仔猪大肠肝菌中,EAEC检出8株、ETEC检出1株、EPEC检出4株、EAEC-EPEC检出1株;共39株.结论:由于猪大肠埃希菌从粪便中排出,导致猪场周围环境造成严重污染,在宰猪的过程中,不正确的储存猪肉方式以及运送阶段,皆会增加猪大肠埃希菌的感染风险.
PCR Analysis of Escherichia coli in Porcine
Objective:To analyze the pathogen carrying status and pathogenic causes of porcine Escherichia coli after PCR detection.Methods:From January to December 2022,97 strains of Escherichia coli were isolated from pig farms,40 strains were isolated from the feces of pregnant sows,and 24 strains were isolated from the feces of weaned piglets.The virulence gene carrying status and pathogenic causes of Escherichia coli in different populations were analyzed.Results:Group A accounted for 42.27%,group B1 accounted for 56.70%,group B2 accounted for 1.03%,group D accounted for 6.19%,group B1 was the dominant group.Among 40 strains of E.coli in pregnant sows,8 strains and 2 strains were detected by EAEC and EHEC.A total of 10 strains were detected.Among 33 strains of E.coli in lactating sows,15 strains were detected by EAEC.Among the 24 strains of Escherichia coli of weaned piglets,8 strains were detected by EAEC,1 strain by ETEC,4 strains by EPEC and 1 strain by EEC-EPEC.A total of 39 strains.Conclusion:Serious pollution of the environment around the pig farm is caused by the excretion of porcine Escherichia coli from the manure.In addition,incorrect storage methods and transportation stages during the slaughter of pigs can increase the risk of porcine Escherichia coli infection.

Escherichia coliPCRanalyze

张琴

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四川国际旅行卫生保健中心,四川 成都 610041

大肠埃希菌 聚合酶链式反应 分析

2024

现代畜牧科技
黑龙江省畜牧研究所

现代畜牧科技

影响因子:0.066
ISSN:2095-9737
年,卷(期):2024.(12)