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牛源无乳链球菌的分离鉴定和毒力基因检测

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该文为监测安徽某规模化奶牛场奶牛乳房炎无乳链球菌的流行情况及其携带的毒力基因,采集该牛场患牛乳样,进行细菌分离培养和PCR鉴定,并进行同源性比较。结果显示,分离株为无乳链球菌,并携带neuC、cfb、cpsM 3种毒力基因。研究结果表明,引起该奶牛乳房炎的主要致病菌为无乳链球菌,为该牛场养殖管理和疾病防控提供基础理论依据。
Isolation,Identification and Virulence Gene Detection of Streptococcus agalactiae from Bovine Origin
This article monitored the prevalence of Streptococcus agalactiae causing mastitis in a large-scale dairy farm in Anhui and the virulence genes carried by these bacteria.Milk samples from affected cows were collected,followed by bacterial isolation,culture,and PCR identification,as well as homology comparison.The results showed that the isolated strains were Streptococcus agalactiae and carried three virulence genes:neuC,cfb,and cpsM.The research results indicate that Streptococcus agalactiae is the main pathogenic bacteria causing mastitis in dairy cows,providing a theoretical basis for the breeding management and disease prevention and control in the dairy farm.

mastitisStreptococcusvirulence gene

左运杰、赵士祥、刘洋洋、齐艳萍

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安徽科技学院动物科学学院,安徽 滁州 239000

乳房炎 链球菌 毒力基因

2024

现代畜牧科技
黑龙江省畜牧研究所

现代畜牧科技

影响因子:0.066
ISSN:2095-9737
年,卷(期):2024.(12)