牛源无乳链球菌的分离鉴定和毒力基因检测
Isolation,Identification and Virulence Gene Detection of Streptococcus agalactiae from Bovine Origin
左运杰 1赵士祥 1刘洋洋 1齐艳萍1
作者信息
- 1. 安徽科技学院动物科学学院,安徽 滁州 239000
- 折叠
摘要
该文为监测安徽某规模化奶牛场奶牛乳房炎无乳链球菌的流行情况及其携带的毒力基因,采集该牛场患牛乳样,进行细菌分离培养和PCR鉴定,并进行同源性比较.结果显示,分离株为无乳链球菌,并携带neuC、cfb、cpsM 3种毒力基因.研究结果表明,引起该奶牛乳房炎的主要致病菌为无乳链球菌,为该牛场养殖管理和疾病防控提供基础理论依据.
Abstract
This article monitored the prevalence of Streptococcus agalactiae causing mastitis in a large-scale dairy farm in Anhui and the virulence genes carried by these bacteria.Milk samples from affected cows were collected,followed by bacterial isolation,culture,and PCR identification,as well as homology comparison.The results showed that the isolated strains were Streptococcus agalactiae and carried three virulence genes:neuC,cfb,and cpsM.The research results indicate that Streptococcus agalactiae is the main pathogenic bacteria causing mastitis in dairy cows,providing a theoretical basis for the breeding management and disease prevention and control in the dairy farm.
关键词
乳房炎/链球菌/毒力基因Key words
mastitis/Streptococcus/virulence gene引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据