Correlation Analysis between TBC1D5 and Multiple Birth Traits in Qira Black Sheep
To investigat the effect of the TBC1D5 gene on multiple births in Qira black sheep.We used direct sequencing of PCR products to scan for SNPs in the gene TBC1D5,and analyzed the correlation between its polymorphic loci and the characteristics of multiple births in Qira black sheep.the results provide a scientific basis for improving the genetic traits of local sheep breeds and promoting their innovative utilization.Correlation analysis shows that g.328889 G>C was significantly correlated with litter size(P=0.03).The mean litter size of females with GG genotype was 0.33 more than that of individuals with GC genotype.This results verified the polymorphism of TBC1D5 gene g.328889 G>C in Qira black sheep,which is of great significance for molecular marker selection and breeding of Qira black sheep.