Rapid detection of Fusarium oxysporum on soybean using recombinase polymerase amplification
The objective of this study is to rapidly detect Fusarium oxysporum on soybeans using recombinase polymerase amplification(RPA)technology through experimental research.Three forward and reverse primers were designed for the specific genes of F.oxysporum,and the optimal primer pairs were selected that can achieve a lower limit of detection at the femtogram level(90 fg/μL)under constant temperature(39℃)for 15 to 20 minutes.There was no cross-reaction with Phytophthora megasperma f.sp.glycine a and Cercospora sojina.Compared to traditional PCR and real-time quantitative PCR,the RPA detection method for Fusarium in soybeans,established in this study,is rapid,convenient,and allows for immediate observation.This provides a new technical means for the rapid detection of this pathogen.
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