Isolation and identification of Ampelomyces quisqualis and its potential as biocontrol agent against powdery mildew
150 powdery mildew-i nfected plant samples of three plant species were collected in the Shihezi region of Xinjiang,and mycoparasitic fungi were isolated.Tested strains of isolates were confirmed by phylogenetic analysis and morphological characteristics study.The result showed that the pycnidia of the isolated mycoparasite fungi were dark brown,multicolonial,(40.66-82.92)μm×(34.65-63.05)μm in size.The conidia were unicellular,hyaline,guttulate at both ends,and the size of(3.88-5.67)μm×(2.19-2.75)μm.The ITS-rDNA sequences of tested strains showed 94％to 100％homology with those of Ampelomyces quisqualis.Therefore,the mycoparasite fungus was identified as A.quisqualis based on its morphological and molecular characterstics.The potential of biparasitic and biocontrol effect against Golovinomyces cichoracearum of the 12 tested A.quisqualis strains were evaluated.It was found that the 12 strains could parasitize on Golovinomyces cichoracearum,and most of the strains had average re-parasitic indexes of 26.21,among which As-81 and Rh-60 had the best control effect against powdery mildew with the respective efficacies of 46.62％and 46.15％,respectively.
Ampelomyces quisqualishyperparasite fungi identificationbiological control
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