Identification and analysis of biotype of fall armyworm in Hainan
Hainan serves as an important stop on the migration route of Spodoptera frugiperda in China.This study analyzed the biotype of S.frugiperda in Hainan by using two molecular markers,CO Ⅰ and Tpi,and carbon stable isotope determination methods.Samples were collected by vertical-pointing searchlight-trap in Sanya,Haikou,and Danzhou from 2020 to 2022.The results showed that 52.08％of the samples were rice-biotype and 47.92％were corn-biotype based on the CO Ⅰ gene sequence.All samples were identified as corn-biotype based on the gTpi183Y site of the fourth exon of the Tpi gene.Further analysis of the gTpi192Y and gTpi198Y sites revealed three subtypes of corn-biotype:Tpi-Cal(accounting for 47.73％),Tpi-Ca2(accounting for 11.36％),and Tpi-Cal/Ca2(accounting for 40.91％).The results of the carbon stable isotope indicated that from 2020 to 2022,the proportion of moths feeding on C4 plants(corn)was 42.86％,57.89％,and 58.33％,while the proportion of moths feeding on both C3(rice)and C4 plants was 57.14％,42.11％,and 33.33％,respectively.Additionally,only 8.33％of the moths sampled in 2022 fed on C3 plants.This study may aid in tracking and monitoring the biotype of S.frugiperda in Hainan and provide a reference for accurate monitoring and early warning.
NETL
NSTL
万方数据
