Effect of different concentrations of chlorine dioxide treatment on tobacco root diseases
The objective of this study is to investigate the impact of soil treated with varying concentrations of chlorine dioxide on the control of tobacco root disease,as well as on leaf characteristics,yield,and quality.Two concentrations of chlorine dioxide,1.5 kg/667m2 and 2.0 kg/667m2,were applied to the fields with moderate and severe root disease.The control efficiency of each treatment on tobacco root disease and tobacco leaf characteristics was measured.Quantitative fluorescent PCR and GC-MS technology were used to detect the number of major pathogenic microorganisms in soil and volatile components of tobacco leaves in different treatments.The economic benefits of each treatment were compared.The results showed that after chlorine dioxide treatment,the incidence rate of heavy disease fields decreased significantly.The disease control effect of 1.5 kg/667m2 treatment and 2.0 kg/667m2 treatment was equal.In the moderately infested field,the incidence rate of disease was the lowest when treated with 2.0 kg/667m2 of chlorine dioxide.Treatment with chlorine dioxide significantly improved the plant height,plant width,leaf number,and other agronomic characteristics of tobacco.Additionally,the yield and value of top-grade and medium-grade tobacco leaves were also improved.Principal component analysis showed significant differences in volatile components between the two treatment groups,and the main beneficial substances of mature tobacco leaves were significantly increased.The results of detecting pathogenic microorganisms in soil under different treatments showed that the use of chlorine dioxide in a certain concentration range had the most significant inhibitory effect on Ralstonia solanacearum and Phytophthora nicotiana in moderately infected circumstances.Various concentrations of chlorine dioxide can effectively control the occurrence of tobacco root diseases and improve the agronomic characteristics of tobacco leaves,thereby increasing yield.