Osmotic sensitivity and membrane permeability of Peyronellaea arachidicola under tebuconazole stress
In this paper,the osmotic pressure sensitivity and membrane permeability of tebuconazole-resistant and sensitive strains of Peyronellaea arachidicola,pathogen of peanut web blotch disease,were compared,focusing on the physiological and biochemical characteristics of tebuconazole-resistant strains.The results indicated that under different concentrations of NaCl,both sensitive and resistant strains showed an initial increase in colony diameter followed by a decrease.Sensitive strains were less affected by low osmotic pressure but were more sensitive to medium and high osmotic pressure environments.In contrast,resistant strains were not sensitive to low and medium osmotic pressure environments,but were sensitive to high osmotic pressure environments.Following treatment with varying concentrations of tebuconazole,the relative permeability of the test strains increased over time to a certain extent.The relative permeability of S-TL-11 and S-JL-20 were lower than those of R-AS-02 and R-LY-72 under each concentration of chemical treatment.It showed that the resistant strains had stronger cell membrane permeability and can excrete more tebuconazole,while the sensitive strains had poorer cell membrane permeability and were more sensitive to tebuconazole.The results will provide a theoretical basis for further exploration of the resistance mechanism of P.arachidicola to tebuconazole and guide the scientific use of fungicides in the field.
peanut web blotch diseasetebuconazoleosmotic pressuremembrane permeability
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