Evaluation of the prevention and control effects of fungicides to rice leaf blast by artificial inoculation method in greenhouse
The prevention and control effects of three commonly used fungicides on rice leaf blast at the seedling-stage were evaluated by artificial inoculation treatments under greenhouse conditions.Magnaporthe oryzae strain HN09-1C-7 was inoculated onto 12 rice monogenic differential lines and the rice cultivar RD23 to evaluate its pathogenicity.M.oryzae strain HN09-1C-7 showed consistent pathogenicity,indicating its suitability for subsequent inoculation experiments.Based on this observation,two experimental groups were designed,one in which fungicides were applied fisrst,followed by inoculation,and another in which inoculation was conducted first,followed by the application of fungicides.The results demonstrated that when the dosages of the effective components of methylthio tricyclazole,isoprothiolane,and trifloxy-strobin·tebuconazole were 378 mUhm2,600 mL/hm2,and 202.5 g/hm2,respectively,the control effects on rice leaf blast were 93.97%,90.06%,and 99.68%in the fungicides application followed by inoculation group,and 41.80%,67.96%,and 74.49%in the inoculation followed by fungicides application group,respectively.It can be concluded that all three tested fungicides exhibited relatively high preventive effects against rice leaf blast.However,the effect was relatively lower after the disease had occurred.This study provides an important reference for the rational use of leaf blast in rice production.
Oryza sativa L.rice leaf blastartificial inoculation treatmentfungicidesprevention and control efficacy