引起黄芪根腐病的锐顶镰刀菌的RPA检测方法

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中文摘要：黄芪根腐病是可由多种镰刀菌引起的土传病害,锐顶镰刀菌为主要病原菌.根据锐顶镰刀菌转录延伸因子(TEF-1α)的特异性区段设计引物,通过优化反应温度、反应时间、灵敏度和特异性试验,以及开展对实际样品的检测,建立了锐顶镰刀菌的的重组酶聚合酶扩增(RPA)检测方法.该方法的最优反应条件为39℃、30 min,能够特异性检测锐顶镰刀菌,检测灵敏度为10 μg/mL;该方法应用于实际样品检测,具有检测速度快、灵敏度高、特异性强的优点,有利于黄芪根腐病的病原监测与病害的早期诊断.

外文标题：Recombinant polymerase amplification method for detection of Fusarium acuminatum causing Astragalus membranaceus root rot

外文摘要：Astragalus membranaceus root rot is a serious soil-borne disease caused by Fusarium spp..Of these,F.acuminatum was found to be the major pathogen associated with this disease.Specific primers for F.acuminatum were designed according to the specific region of transcription elongation factor-1α(TEF-1α).A recombinant enzyme polymerase amplification(RPA)detection method was developed based on reaction temperature and time optimization,sensitivity and specificity testing,and the detection of diseased samples.The results showed that the optimal reaction conditions for this method were 39 ℃ for 30 min,which enabled the specific detection of F.acuminatum,and the detection sensitivity could reach 10 μg/mL.This RPA method could be used for the detection of infected samples in the field,which had the advantages of a rapid detection speed,high sensitivity and strong specificity,and was conducive to the pathogen surveillance and early diagnosis of A.membranaceus root rot.

外文关键词：

Astragalus membranaceus root rotFusarium acuminatumRecombinase polymerase amplificationmolecular detection

作者：

王春伟、李广信、周书悦、左敏、王琳、苏健、王燕

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作者单位：

山西农业大学植物保护学院,山西太谷 030801

山西农业大学农学院,山西太原 030031

太原海关,山西太原 030021

关键词：

黄芪根腐病锐顶镰刀菌重组酶聚合酶扩增分子检测

基金：

国家重点研发计划山西农业大学学术恢复科研专项项目山西省现代农业产业技术体系建设专项资金山西农业大学科技创新基金项目山西农业大学科技创新基金项目

项目编号：

2019YFC17108002020xshf052021-112016YJ112016YJ12

出版年：

2024

中国植保导刊

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中国植保导刊

北大核心

影响因子：0.679

ISSN：1672-6820

年,卷(期)：2024.44(9)