摘要
马铃薯病毒病是影响马铃薯块茎产量和品质的主要限制因素之一.在恩施州7个马铃薯主栽县市种薯生产基地采集了 305份具有典型症状的马铃薯样品,应用DAS-ELISA法进行检测,筛查PVX、PVY、PLRV、PVA、PVS和PVM等6种主要病毒,其中,PVS、PVM和PVA的检出率分别达25.90％、23.61％和22.95％,是该地区马铃薯携带的主要病毒;PVX和PLRV在低山区(海拔≤700 m)最为普遍,检出率均为19.44％;PVM在二高山区(海拔700～1 000 m)最为普遍,检出率为40.79％;PVS在高山区(海拔≥1 000 m)最为普遍,检出率为31.09％.马铃薯种薯种植区域以流行 1种和2种病毒最为普遍,单一病毒中以PVS的检出率最高,2种病毒以PVS+PVA和PVS+PVM检出率最高,3种和4种病毒的流行情况并不普遍.
Abstract
Potato virus disease is one of the main limiting factors affecting the yield and quality of potato tubers.In this study,305 potato samples with typical symptoms were collected from seven potato production bases in Enshi Prefecture.The DAS-ELISA method was used for the detection and screening of six major viruses(PVX,PVY,PLRV,PVA,PVS,and PVM).The results are presented as follows:(1)PVS,PVM,and PVA were detected in 25.90％,23.61％,and 22.95％of sampes,respectively,and were the main viruses carried by seed potatoes in this area.(2)PVX and PLRV were the most common in low-altitude mountainous areas(altitude ≤700 m),with a detection rate of 19.44％.PVM was the most prevalent in middle-altitude areas(700～1 000 m),with a detection rate of 40.79％.PVS was the most common in high-altitude areas(≥1 000 m),with a detection rate of 31.09％.(3)The majority of seed potatoes were found to carry a single virus or both viruses.The detection rate of PVS was the highest in single virus infections,while the detection rates of PVS+PVA and PVS+PVM were the highest in two-virus infections.The prevalence of three or four viruses was not common.
基金项目
湖北省自然科学基金联合项目(2023AFD064)
现代农业产业技术体系专项资金资助(CARS-09)
湖北省中央引导地方科技发展专项(2022BGE268)
NETL
NSTL
万方数据