Study on the changes in enzyme activity of quinclorac-resistant barnyard grass in paddy fields
Quinclorac was a specific herbicide used to control barnyard grass in rice fields,but its continued use has led to increasing resistance of barnyard grass to it.In this paper,the resistance mechanism of barnyard grass to quinclorac was investigated,focusing on changes in enzyme activity.It was found that the greater the activity of CAT enzyme(catalase),the greater the resistance of barnyard grass to quinclorac.Following quinclorac stress,the activity of GST enzyme decreased.However,the decline in GST enzyme activity was less pronounced in the high-resistant barnyard grass population compared to the sensitive population.The changes in gene expression of related enzymes showed that the expression of CAT initially increased and subsequently decreased.The increase of CAT expression in the high-resistant type was significantly higher than that observed in the sensitive type,indicating that the higher the gene expression of CAT enzyme,the greater the resistance of the barnyard grass to quinclorac.The expression of GST enzyme showed a pattern of first decreasing and then increasing.The change in GST enzyme expression in the high-resistant type was significantly lower than that in the sensitive type,indicating that the higher the expression of GST enzyme,the stronger the resistance of barnyard grass to quinclorac.This study provides a theoretical and practical basis for the integrated management of barnyard grass.
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