Pathogen identification of walnut black spot and laboratory evaluation of disease resistance in different walnut cultivars
This study aimed to clarify the pathogen of walnut black spot in Wenxian,Henan Province,and the disease resistance of different walnut cultivars,a representative bacterium,WB-Wen2,with high isolation frequency and strong pathogenicity on local walnut fruit,was employed as the subject of investigation.Identification was achieved through the colony morphology observation and gyrB gene sequence alignment analysis.The fruits of 20 major cultivars were collected for laboratory resistance evaluation.The results showed that the pathogen of the walnut black spot in Wenxian County was identified as Pantoea agglomerans.Only three cultivars,namely Yuanlin,Zhonglin 1,and Zhonglin 5,exhibited resistance to the disease,while the remaining 17 cultivars demonstrated susceptibility to the disease,which requires attention to the control of walnut black spot during the late stage of fruit growth.
walnut black spotPantoea agglomeransdisease resistance,cluster analysis