核桃黑斑病病原鉴定及不同核桃品种的抗病性评价
Pathogen identification of walnut black spot and laboratory evaluation of disease resistance in different walnut cultivars
吴曼曼 1孙书豪 2王霞 3朱文亭 2周晨霞 2张建雨 2仵春 4孙炳剑 2卢绍辉5
作者信息
- 1. 河南农业大学植物保护学院,河南郑州 450046;河南省林业科学研究院,河南郑州 450002
- 2. 河南农业大学植物保护学院,河南郑州 450046
- 3. 河南省长垣市林业局,河南长垣 453400
- 4. 河南省产品质量检验技术研究院,河南郑州 450000
- 5. 河南省林业科学研究院,河南郑州 450002
- 折叠
摘要
为了明确河南省温县核桃黑斑病的病原菌种类以及不同核桃品种对该病的抗性,以当地核桃病果上分离频率较高且具强致病性的代表性细菌(菌株编号WB-Wen2)为对象,结合菌落形态观察与gyrB基因序列比对分析进行鉴定,并采集20个核桃品种的果实,在室内开展抗病性评价.经鉴定,温县核桃黑斑病病原为成团泛菌(Pantoea agglomerans).通过对接种后果实上的病斑直径进行系统聚类分析,20个品种中仅元林、中林1号、中林5号表现抗病,其余17个品种均表现感病,在果实生长后期需注意防控.
Abstract
This study aimed to clarify the pathogen of walnut black spot in Wenxian,Henan Province,and the disease resistance of different walnut cultivars,a representative bacterium,WB-Wen2,with high isolation frequency and strong pathogenicity on local walnut fruit,was employed as the subject of investigation.Identification was achieved through the colony morphology observation and gyrB gene sequence alignment analysis.The fruits of 20 major cultivars were collected for laboratory resistance evaluation.The results showed that the pathogen of the walnut black spot in Wenxian County was identified as Pantoea agglomerans.Only three cultivars,namely Yuanlin,Zhonglin 1,and Zhonglin 5,exhibited resistance to the disease,while the remaining 17 cultivars demonstrated susceptibility to the disease,which requires attention to the control of walnut black spot during the late stage of fruit growth.
关键词
核桃黑斑病/成团泛菌/品种抗病性/聚类分析Key words
walnut black spot/Pantoea agglomerans/disease resistance,cluster analysis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据