Pathogen identification of canker disease of Aesculus chinensis Bunge and preliminary screening of control agents
In recent years,seven-leaf trees(Aesculus chinensis Bunge)in Zizhuyuan Park of Beijing have emerged with brown spots on their branches and trunks.Some trees were severely affected,with bark splitting and shedding,leading to significant weakness and even death.The investigation was conducted in Zizhuyuan Park,where the typical symptoms of the disease were observed and recorded,and samples were collected.The isolated fungi were identified using both morphological and molecular biological methods,which revealed that the pathogen was Fusarium oxysporum.On this basis,different dilution concentrations of tetramycin,mancozeb,octylamine acetate,Trichoderma harzianum+Bacillus subtilis,and Bacillus velezensis were used for preliminary screening tests in the laboratory.Healthy branches of A.chinensis were inoculated and the amplification of lesions on the branches of each treatment was observed.It was found that the combination of Trichoderma harzianum and Bacillus subtilis had the best control effect,with no expansion of lesion area observed following inoculation.The relative control effect was 100％.The new lesion area of branches treated with Bacillus velezensis was only 0.13 mm?,and the relative control effect was 98.75％.These findings provide a theoretical reference for the prevention and control of Aesculus canker disease.
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