Regular screening for HPV(Human Papilloma Virus,HPV)is of great significance in improving women's health and preventing the occurrence of cervical cancer.In order to improve the automation of biochemical detection,reduce manual intervention and minimize the misjudgment,it is very important in designing an HPV biochip reading device.Based on this object,the application the STM32F429 processor combined with the OV2640 CMOS sensor,equipped with an M12 lens and a movable 30 mm stage,to achieve the HPV biochip reading device,which can fully leverage the screening advantages of visible and color TIB-23 HPV biochips.The test results show that the designed HPV biochip reading device can directly apply the biochemical reaction tray of HPV hybridization instrument,and successfully read the quadruple TIB-23 HPV biochips.This is of great significance for improving the automation level of HPV typing screening.