The relationship between activated platelet-CD4+T cell aggregates and the immune status of HIV/AIDS patients
Objective To explore the proportion of activated platelet-CD4+T cell aggregates in the peripheral blood of HIV/AIDS patients in different disease states,and to investigate the reasons for the aggregation of activated platclct-CD4+cell aggregates and their relationship with disease progression.Methods A cohort of 100 treatment-naïve HIV/AIDS patients(TN),18 immunological responders(IR)post-combination antiretroviral therapy(cART),13 immunological non-responders(INR),and 20 healthy controls(HC)were enrolled.Flow cytometry was used to determine the proportion of activated platelet-CD4+T cell aggregates and the self-activation levels in both platelets and CD4+T cells,with the aim of investigating changes in the proportion of activated platelet-CD4+T cell aggregates,their relationship with disease progression,and the influencing factors involved in aggregate formation.Results The proportion of activated platelet-CD4+T cell aggregates was significantly higher in the TN group(TN vs.HC:median 6.420 vs.5.200,Z=2.093,P=0.033 2),and this proportion was negatively correlated with CD4+T cell count and the CD4+/CD8+ratio(CD4+T count,r=-0.553 0,P＜O.OO0 1;CD4+/CD8+ratio,r=-0.601 5,P＜0.000 1),and positively correlated with viral load(r=0.329 8,P=0.001 0).After cART,the proportion of activated platelet-CD4+T cell aggregates was significantly lower in the IR group than in the TN group(IR vs.TN:median 4.535 vs.6.420,Z=2.776,P=0.005 3),whereas no reduction was observed in the INR group(INR vs.TN:median 6.730 vs.6.420,Z=0.654 2,P=0.511 2).The proportion of activated platelet-CD4+T cell aggregates in the peripheral blood of the TN group was positively correlated with the proportion of HLA-DR+CD4+T cells(r=0.426 2,P=0.005 5),but showed no correlation with the platelet count and platelet activation level.Conclusions The proportion of activated platelet-CD4+T cell aggregates is closely related to the disease progression in HIV/AIDS patients and the efficacy of cART,and the formation of these aggregates is primarily influenced by the activation level of CD4+T cells.
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