Characterization of cross-regional transmission on the predominantly endemic CRF07_BC strain in China through the sequence of the pol gene region of HIV-1
Objective In order to provide data support for the promotion of precise HIV prevention and control,this article analyze the cross-regional transmission characteristics of the CRF07_BC strain in China,by constructing a molecular network of the sequence of the pol gene region of the CRF07_BC strain of HIV-1 in China.Methods FastTree and the subtyping tool of the China HIV Genetic Sequence Data Platform(https://nmdc.cn/hiv/)were used to discriminate subtypes,and the pol gene sequences of the CRF07_BC strain in China were screened to construct a molecular network.Univariate and multivariate logistic regression analysis were used to identify the factors influencing access rate and cross-regional connectivity.Multi-jurisdictional analysis indexes,such as cross-region clustering coefficients,were calculated to characterize the transmission connectivity and intensity among different provinces.Results A total of 81 612 sequences of the pol gene region of HIV-1 strains in China were collected,including 26 181 sequences of CRF07_BC strain.Under the 0.5％gene distance threshold,2 028 molecular networks were constructed.The largest molecular network,named 07-1-1,containing 6 262 nodes from 31 provinces(autonomous regions and municipalities)nationwide,and the predominant transmission route was through male-to-male sex behavior(32.0％,2 005/6 262).The indicator of multi-jurisdictional transmission analysis showed that 68.9％of the molecular networks in 07-1-1 were multi-jurisdictional connecting edges,and 80.6％were multi-jurisdictional transmission nodes.The multi-jurisdictional clustering coefficients showed that the multi-jurisdictional transmission of molecular network 07-1-1 occurs mainly in Guangdong Province(66.6％,79 114/118 819)and Beijing Municipality(27.9％,33 159/118 819),as well as in other regions of China,including the east,southwest,south,and central regions.Conclusions The CRF07_BC strain plays a dominant role in the multi-jurisdictional transmission of HIV in China,with Guangdong Province and Beijing Municipality at the center of the multi-jurisdictional transmission.Effectively controlling the multi-jurisdictional transmission of HIV strains in China will be one of the most important initiatives for the high-quality development of AIDS prevention and control in China.
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万方数据
