浦东新区HIV-1不同亚型感染者的肠道菌群特征及其与血脂异常的关联
Characterization of intestinal flora in various genetic subtypes of HIV-infected patients and its association with dyslipidemia in Pudong New Area,China
张浩然 1陈盼盼 2陈婧 3马铭君 1邵鹏 3周素娟 4肖绍坦 5何纳 4辛辛5
作者信息
- 1. 大理大学公共卫生学院,云南大理 671000;上海市浦东新区疾病预防控制中心/复旦大学浦东预防医学研究院,上海 200136
- 2. 上海市浦东新区疾病预防控制中心/复旦大学浦东预防医学研究院,上海 200136;复旦大学公共卫生学院,上海 200032
- 3. 上海市浦东新区疾病预防控制中心/复旦大学浦东预防医学研究院,上海 200136
- 4. 复旦大学公共卫生学院,上海 200032
- 5. 大理大学公共卫生学院,云南大理 671000;上海市浦东新区疾病预防控制中心/复旦大学浦东预防医学研究院,上海 200136;复旦大学公共卫生学院,上海 200032
- 折叠
摘要
目的 了解浦东新区不同基因亚型的HIV感染者肠道菌群的差异及其与血脂指标之间的关系.方法 收集HIV感染者(HIV+组)和年龄、BM1相匹配的HIV阴性人群(HIV-组)的粪便样本,进行16SrDNA测序,并对HIV+组进行基因亚型测定,同时测定外周血血脂水平并进行生物信息学分析,采用Spearman秩相关分析肠道菌群-血脂指标相关性.结果 纳入HIV+组和HIV-组各47例,均为男性,其中HIV感染者基因亚型包括CRF01_AE组(25例)和CRF07_BC组(22例).CRF01_AE组的血脂指标HDL-C异常情况高于CRF07_BC组(P＜0.05).HIV+组和HIV-组的β多样性差异有统计学意义(R2=0.034 6,P＜0.05).在CRF07_BC组中观察到来自厚壁菌门的5种肠道细菌富集(LDA均＞2.0),包括gauvreauii瘤胃球菌属、Dorea属、颤杆菌克属、CAG_56菌属、乳球菌属(P均＜0.05).HIV感染者的HDL-C水平和Dorea菌呈正相关(r=0.302,P=0.039).结论 不同基因亚型的HIV感染者的肠道菌群特征存在差异,HDL-C异常情况与差异菌种存在相关性,还需进一步的研究.
Abstract
Objective To investigate the differences in intestinal flora among HIV-infected patients with various genotypes and their correlation with blood lipid indices in Pudong New Area.Methods Stool samples were collected from HIV-infected patients(HIV+group)and age-BMI-matched HIV-negative individuals(HIV-group)for 16S rDNA sequencing,and the genetic subtype were tested in HIV+group.Additionally,lipid levels in peripheral blood were quantitatively determined and subsequently analyzed bioinformatically.Spearman's rank order correlation was employed to analyze the correlations between intestinal flora and lipid indices.Results A total of 47 HIV-infected patients and 47 HIV-negative individuals were included,all of whom were men.The HIV-infected genetic subtypes comprised the CRF01_AE group(25 patients)and the CRF07_BC group(22 patients).The CRF01_AE group exhibited higher abnormalities in the lipid index,specifically high-density lipoprotein cholesterol(HDL-C),than the CRF07_BC group(P＜0.05).Statistical differences in β diversity were observed between HIV+group and HIV-group(R2=0.0346,P＜0.05).Enrichment of five intestinal bacteria from the phylum Firmicutes was noted in the CRF07_BC group(LDA all＞2.0).These included Ruminococcus gauvreauii,Dorea,Oribacterium,CAG_56,and Lactococcus(P all＜0.05).A positive correlation was identified between Dorea and HDL-C levels in HIV-infected patients(r=0.302,P=0.039).Conclusions There are differences in the gut flora characteristics of HIV-infected patients with different genetic subtypes and a correlation between HDL-C abnormalities and differential strains exists,necessitating further study.
关键词
艾滋病病毒/基因亚型/肠道菌群/血脂异常Key words
HIV/genetic subtype/gut microbiota/dyslipidemia引用本文复制引用
基金项目
浦东新区卫生系统学科带头人培养计划(PWRd2022-01)
浦东新区卫生系统医学学科建设项目(PWYgts2021-04)
上海市浦东新区卫生健康委卫生科技项目(PW2018B-41)
浦东新区科技发展基金民生科研专项(PKJ2023-Y71)
出版年
2024
NETL
NSTL
万方数据