Characterization of intestinal flora in various genetic subtypes of HIV-infected patients and its association with dyslipidemia in Pudong New Area,China
Objective To investigate the differences in intestinal flora among HIV-infected patients with various genotypes and their correlation with blood lipid indices in Pudong New Area.Methods Stool samples were collected from HIV-infected patients(HIV+group)and age-BMI-matched HIV-negative individuals(HIV-group)for 16S rDNA sequencing,and the genetic subtype were tested in HIV+group.Additionally,lipid levels in peripheral blood were quantitatively determined and subsequently analyzed bioinformatically.Spearman's rank order correlation was employed to analyze the correlations between intestinal flora and lipid indices.Results A total of 47 HIV-infected patients and 47 HIV-negative individuals were included,all of whom were men.The HIV-infected genetic subtypes comprised the CRF01_AE group(25 patients)and the CRF07_BC group(22 patients).The CRF01_AE group exhibited higher abnormalities in the lipid index,specifically high-density lipoprotein cholesterol(HDL-C),than the CRF07_BC group(P＜0.05).Statistical differences in β diversity were observed between HIV+group and HIV-group(R2=0.0346,P＜0.05).Enrichment of five intestinal bacteria from the phylum Firmicutes was noted in the CRF07_BC group(LDA all＞2.0).These included Ruminococcus gauvreauii,Dorea,Oribacterium,CAG_56,and Lactococcus(P all＜0.05).A positive correlation was identified between Dorea and HDL-C levels in HIV-infected patients(r=0.302,P=0.039).Conclusions There are differences in the gut flora characteristics of HIV-infected patients with different genetic subtypes and a correlation between HDL-C abnormalities and differential strains exists,necessitating further study.
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