Effect of Jianaikang concentrated pill combined with cART on the intestinal and intestinal flora of patients with HIV
Objective To study the effect of Jianaikang concentrated pill combined with antiretroviral therapy(cART)on the intestinal tract of patients with HIV.Methods A total of 26 patients with HIV were enrolled.13 patients in the test group were administered Jianaikang concentrated pill+cART,and 13 patients in the control group received Jianaikang concentrated pill placebo+cART,and all the patients were treated for 48 weeks.The blood and stool of the two groups were collected and analyzed by cytological examination,16SrRNA high-throughput sequencing,and short-chain fatty acids and intestinal mucosal injury markers.Results In the test group,the post-treatment expression level of CD4+CD45RO+(269.77±57.78)was higher than at pre-treatment(178.46±83.26)(P＜0.01).Additionally,the post-treatment expression level of CD4+CCR5+(19.08±5.95)was higher than at pre-treatment(10.46±9.98)(P＜0.05).Conversely,the post-treatment CD4+CXCR4+(174.77±63.23)was lower than that at pre-treatment(244.46±89.38)(P＜0.05).In the control group,the post-treatment CD4/CD8 ratio(0.81±0.37)was higher than at pre-treatment(0.52±0.25)(P＜0.05),and the post-treatment CD4+CD45RO+(313.15±95.04)was higher than at pre-treatment(195.38±77.56)(P＜0.01).At the genus level,the proportions of Pseudomonas,Weissella,Catenibacterium,and Actinomyces in the test group were higher than those in the control group(P＜0.05),whereas the proportion of Tyzzerella 4 in the test group was lower than that in the control group(P＜0.05).The levels of all short-chain fatty acids,except for acetic acid,in the test group were lower than those in the control group(P＞0.05),and the levels of all peripheral intestinal mucosal injury markers in the test group were lower than those in the control group(P＞0.05).Conclusions After 48 weeks of treatment,the intestinal tract of the two groups of patients with HIV exhibited distinct differences.Compared with cART alone,combined treatment with Jianaikang concentrated pill produced a synergistic effect on immune reconstruction.Combination therapy can potentially increase the proportion of probiotics and showed a trend toward reducing the risk of intestinal mucosal injury.
NETL
NSTL
万方数据
