Objective To study the effect of Jianaikang concentrated pill combined with antiretroviral therapy(cART)on the intestinal tract of patients with HIV.Methods A total of 26 patients with HIV were enrolled.13 patients in the test group were administered Jianaikang concentrated pill+cART,and 13 patients in the control group received Jianaikang concentrated pill placebo+cART,and all the patients were treated for 48 weeks.The blood and stool of the two groups were collected and analyzed by cytological examination,16SrRNA high-throughput sequencing,and short-chain fatty acids and intestinal mucosal injury markers.Results In the test group,the post-treatment expression level of CD4+CD45RO+(269.77±57.78)was higher than at pre-treatment(178.46±83.26)(P＜0.01).Additionally,the post-treatment expression level of CD4+CCR5+(19.08±5.95)was higher than at pre-treatment(10.46±9.98)(P＜0.05).Conversely,the post-treatment CD4+CXCR4+(174.77±63.23)was lower than that at pre-treatment(244.46±89.38)(P＜0.05).In the control group,the post-treatment CD4/CD8 ratio(0.81±0.37)was higher than at pre-treatment(0.52±0.25)(P＜0.05),and the post-treatment CD4+CD45RO+(313.15±95.04)was higher than at pre-treatment(195.38±77.56)(P＜0.01).At the genus level,the proportions of Pseudomonas,Weissella,Catenibacterium,and Actinomyces in the test group were higher than those in the control group(P＜0.05),whereas the proportion of Tyzzerella 4 in the test group was lower than that in the control group(P＜0.05).The levels of all short-chain fatty acids,except for acetic acid,in the test group were lower than those in the control group(P＞0.05),and the levels of all peripheral intestinal mucosal injury markers in the test group were lower than those in the control group(P＞0.05).Conclusions After 48 weeks of treatment,the intestinal tract of the two groups of patients with HIV exhibited distinct differences.Compared with cART alone,combined treatment with Jianaikang concentrated pill produced a synergistic effect on immune reconstruction.Combination therapy can potentially increase the proportion of probiotics and showed a trend toward reducing the risk of intestinal mucosal injury.
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