Objective To understand the awareness of hepatitis C prevention and control knowledge among healthcare professionals in Baoshan City,in order to provide a theoretical basis for improving the ability of healthcare professionals to prevent and control hepatitis C.Methods An electronic questionnaire survey was conducted using the cluster sampling method from October 2022 to January 2023 among healthcare professionals from healthcare institutions within the jurisdiction of Baoshan City.Logistic regression model was performed to analyze the factors influencing the awareness rate of hepatitis C prevention and treatment knowledge among healthcare professionals.Results A total of 2 509 valid questionnaires were collected.The overall awareness rate of hepatitis C prevention and treatment knowledge stood at 24.31%.Specifically,the awareness rate for basic knowledge reached 52.33%,for hepatitis C diagnosis knowledge it was 24.43%,for hepatitis C treatment knowledge 66.72%,and for hepatitis C protection knowledge 27.54%.Healthcare professionals with a bachelor's degree(OR=2.543,95%CI:1.360-4.753),master's degree or above(OR=2.820,95%CI:1.186-6.703),intermediate title(OR=1.382,95%CI:1.063-1.797),senior title(OR=1.822,95%CI:1.229-2.701),and employed in secondary hospitals(OR=1.862,95%CI:1.380-2.512)and tertiary hospitals(OR=1.307,95%CI:1.012-1.688)had a higher overall awareness of hepatitis C prevention and treatment knowledge.Conclusions The awareness rate of hepatitis C prevention and control knowledge among healthcare professionals in Baoshan City is relatively low,with a lack of in-depth learning and mastery of knowledge about hepatitis C diagnosis,treatment,and protection.In future training on the prevention and treatment of hepatitis C,emphasis should be placed on individuals with low educational backgrounds,those with junior or lower professional titles,and healthcare professionals in primary healthcare institutions.The training coverage should be expanded to ensure full coverage for non-hepatology healthcare professionals.