Diversity and correlation analysis of baseline oral flora among HIV-infected patients with different immune reconstitution status
Objective This study aimed to investigate the differences in baseline oral microbiota among individuals infected with HIV with different immune reconstitution statuses and to analyze the correlation between these differences and immune reconstitution outcomes.Methods From August 2017 to October 2019,patients from Beijing You'an Hospital,Capital Medical University,and healthy volunteers were enrolled as research participants.Oral saliva samples were collected and subjected to 16S rRNA sequencing.Follow-up visits were conducted for individuals with HIV after 2 years of combined antiretroviral therapy(cART),and they were grouped based on CD4+T cell counts.Differential and correlation analyses were performed using R software.Results A total of 43 males infected with HIV and 15 healthy controls(HC)were included.Among the individuals with HIV,9 were classified into the immunological non-responder(INR)group,9 into the intermediate(M)group,and 25 into the immunological responder(IR)group,based on CD4+T cell counts after 2 years of cART.No significant differences were observed in age among the four groups(P=0.556).Diversity analysis of saliva samples showed statistically significant differences in β-diversity among the INR,M,IR,and HC groups(P=0.003),with increased microbial richness in the IR group.At the genus level,the top five dominant genera distribution differed between the INR,IR,and HC groups.The enrichment of Treponema_2 and Aggregatibacter genera in the IR group was positively correlated with increased CD4+T cell counts and CD4/CD8 cell ratios.In contrast,enrichment of the Solobacterium genus in the INR group was negatively correlated with increased CD4+T cell counts.Receiver operating characteristic curve analysis demonstrated that Treponema_2 had high accuracy in distinguishing between the INR and IR states.Conclusions Significant differences in the baseline oral microbiota were observed among individuals with HIV and different immune reconstitution statuses.Specific characteristics of oral microbiota and marker genera(such as Treponema_2)may play important roles in immune reconstitution in individuals infected with HIV.
HIV infectionimmune reconstitutionoral floradiversity16S rRNA
万方数据
维普
