Current status and genotype analysis of Enterocytozoon bieneusi co-infected in HIV/AIDS patients in Nanchang
Objective This study aimed to investigate the current status and genotypes of Enterocytozoon bieneusi infections to provide evidence for treating patients with HIV/AIDS.Methods Fecal samples were collected from patients with HIV/AIDS at three designated hospitals for AIDS in Nanchang.Nested polymerase chain reaction was used to amplify the ribosomal RNA internal transcribed spacer gene of Enterocytozoon bieneusi,and the suspected products were subjected to bidirectional sequencing and alignment.The evolutionary tree was constructed using MEGA 11.0 software based on the neighbor-joining method.The genotypes of Enterocytozoon bieneusi were identified,and statistical analysis was performed using SPSS 22.0.Results A total of 382 patients with HIV/AIDS were surveyed,and six tested positive for Enterocytozoon bieneusi,resulting in an infection rate of 1.6％(6/382).All six patients were men.No significant differences were observed in the infection rates according to sex,age,occupation,educational level,dietary habits,and hygiene practices(P＞0.05).The phylogenetic tree based on the genetic sequences of the six Enterocytozoon bieneusi positive samples revealed that all the genotypes belonged to Group 1,indicating a strong potential for co-infection.Conclusions Enterocytozoon bieneusi of Group 1 is detected among HIV/AIDS in Nanchang and surveillance should be strengthened.
万方数据
维普
