Analysis of HCV genotypes and resistance associated substitutions to NS5B polymerase inhibitors among the sentinel surveillance population in a city from Gansu Province
Objective To explore the establishment of a resistance associated substitutions(RASs)detection method for hepatitis C virus(HCV)NS5B region,and its preliminary application among a hepatitis C sentinel surveillance population in a city of Gansu Province.Methods Individuals who tested positive for HCV antibodies during the 2020 hepatitis C sentinel surveillance in a city in Gansu Province were included in the study.RNA was extracted from plasma samples,and after reverse transcription and nested PCR amplification,Sanger sequencing was performed for HCV subtyping and analysis of RASs in the NS5B region.Results Out of 74 samples,20 were successfully amplified and sequenced.Four HCV genotypes were identified,with a composition ratio,descending from highest to lowest,of 3a(12/20,60％),1b(4/20,20％),2a(3/20,15％),and 3b(1/20,5％).The RASs for genotype 1b included C316 N(4/4),M414L(1/4),L392I(1/4),I424V(1/4),and V499A(1/4);for genotype 2a,A421V(3/3);and for genotype 3a,A150V(9/12).No RASs were detected for genotype 3b.Direct-acting antiviral agents that have been found to be associated with RASs in this study included sofosbuvir,daclatasvir,and beclabuvir.Conclusions Sentinel surveillance in a city in Gansu Province found that HCV antibody-positive individuals have diverse HCV subtypes,with the common presence of RASs for NS5B polymerase inhibitors.The types of RASs that occur in different NS5B subtypes are different,with the 1b subtype having the greatest diversity of RASs.
hepatitis C virus(HCV)direct antiviral agentsNS5B polymerase inhibitorresistance associated substitutions(RASs)Sanger sequencing
万方数据
维普
