Objective To understand the pathogen composition of hand,foot and mouth disease(HFMD)in a hospital in Kunming,Yunnan province in 2022,and to provide some scientific basis for the prevention and control of HFMD.Methods A total of 407 stool samples were collected from children diagnosed with HFMD in a hospital in Kunming from June to September 2022.The VP1 coding region of enterovirus(EV)was amplified by RT-PCR and EV positive strains were sequenced.Three hundred and four The clinical and pathogenic characteristics were analyzed using software such as SPSS 11.5,enterovirus genotyping and Mega 6.06.Results A total of 407 specimens(74.69％),were positive for EV.A total of 13 genotypes were detected.Coxsackievirus A6(CVA6)was the most frequently detected in the EV(42.43％,129/304),followed by CVA16(35.53％,108/304),CVA5(11.84％,36/304),CVA12(2.30％,7/304),CVA2(1.97％,6/304),CVA10(1.31％,4/304).CVA4,coxsackievirus B5(CVB5)and echovirus 11(E11)accounted for 0.99％(3/304),CVB2 for 0.66％(2/304),and CVA8,E3 and enterovirus A71(EV-A71)were 0.33％(1/304).Among them,CVA6 and CVA16 belonged to B1a and D3a genotypes,respectively.Conclusion HFMD is caused by 13 enteroviruses in a hospital in Kunming city in 2022,of which CVA6 and CVA16 are the predominant pathogens and belonged to the epidemic strains in China.
Hand,foot and mouth diseaseEtiologyEnterovirusCoxsackievirus A6Coxsackievirus A16Phyloge-netic analysis
NETL
NSTL
万方数据
