Develope Monoclonal Antibody against Foot-and-mouth Disease Virus A Type

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外文摘要：In order to develop an anti-FMDV A Type monoclonal antibo by (mAb),BABL/c mice were immunized with FMDV A type.Monoclonal antibodies (mAbs) 7B11 and 8H4 against Foot-and-mouth disease virus (FMDV) serotype A were produced by fusing SP2/O myeloma cells with splenocyte from the mouse immunized with A/AV88.The microneutralization titer of the mAbs 7B11 and 8H4 were 1024 and 512,respectively.Both mAbs contain kappa light chains,the mAbs were IgG1.In order to define the mAbs binding epitopes,the reactivity of these mAbs against A Type FMDV,were examined using indirect ELISA,the result showed that both mAbs reacted with A Type FMDV.These mAbs may be used for further vaccine studies,diagnostic methods,prophylaxis,etiological and immunological research on FMDV.Characterization of these ncindicated that prepared anti-FMDV A mAbs had no cross-reactivity with Swine Vesicular Disease (SVD) or FMDV O,Asial and C Type antigens.Their titers in abdomen liquor were 1:5×106 and 1:2×106,respectively.7B11 was found to be of subtype IgG1,8H4 was classified as IgG2b subtype.The mAbs prepared in this study,are specific for detection of FMDV serotype A,and is potentially useful for pen-side diagnosis.

外文关键词：

Foot-and-mouth disease virus (FMDV) A TypeMonoclonal antibody (mAb)Neutralizing activity

作者：

Tong Lin、Jing Li、Jun-jun Shao、Guo-zheng Cong、Jun-zheng Du、Shan-dian Gao、Hui-yun Chang

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作者单位：

State Key Laboratory of Veterinary Etiological Biology,National Foot and Mouth Disease Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China

基金：

State Key Projects of Transgene ProgramState Key Projects of Transgene ProgramState Key Projects of Transgene Program

项目编号：

2011ZX08011-0042009ZX 08007-008B2009ZX08006-002B

出版年：

2011

DOI：

10.1007/s12250-011-3199-4

中国病毒学

中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD

影响因子：0.393

ISSN：1674-0769

年,卷(期)：2011.26(4)

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