首页|Zika virus non-structural protein 4B interacts with DHCR7 to facilitate viral infection

Zika virus non-structural protein 4B interacts with DHCR7 to facilitate viral infection

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Zika virus(ZIKV)evolves non-structural proteins to evade immune response and ensure efficient replication in the host cells.Cholesterol metabolic enzyme 7-dehydrocholesterol reductase(DHCR7)was recently reported to impact innate immune responses in ZIKV infection.However,the vital non-structural protein and mechanisms involved in DHCR7-mediated viral evasion are not well elucidated.In this study,we demonstrated that ZIKV infection facilitated DHCR7 expression.Notably,the upregulated DHCR7 in turn facilitated ZIKV infection and blocking DHCR7 suppressed ZIKV infection.Mechanically,ZIKV non-structural protein 4B(NS4B)interacted with DHCR7 to induce DHCR7 expression.Moreover,DHCR7 inhibited TANK-binding kinase 1(TBK1)and interferon regulatory factor 3(IRF3)phosphorylation,which resulted in the reduction of interferon-beta(IFN-β)and interferon-stimulated genes(ISGs)productions.Therefore,we propose that ZIKV NS4B binds to DHCR7 to repress TBK1 and IRF3 activation,which in turn inhibits IFN-β and ISGs,and thereby facilitating ZIKV evasion.This study broadens the insights on how viral non-structural proteins antagonize innate immunity to facilitate viral infection via cholesterol metabolic enzymes and intermediates.

7-Dehydrocholesterol reductase(DHCR7)Interferon regulatory factor 3(IRF3)Interferon-beta(IFN-β)Non-structural protein 4B(NS4B)TANK-Binding kinase 1(TBK1)Zika virus(ZIKV)

Weijie Chen、Yukun Li、Xiuling Yu、Zhenwei Wang、Wenbiao Wang、Menglan Rao、Yongkui Li、Zhen Luo、Qiwei Zhang、Jinbiao Liu、Jianguo Wu

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Guangdong Provincial Key Laboratory of Virology,Institute of Medical Microbiology,Jinan University,Guangzhou,510632,China

Foshan Institute of Medical Microbiology,Foshan,528315,China

Halison International Peace Hospital,Hebei Medical University,Hengshui,053000,China

Medical Research Center,Guangdong Provincial People'ss Hospital,Guangdong Academy of Medical Sciences,Guangzhou,510080,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaGuangdong Basic and Applied Basic Research Foundation

81730061818020082021A1515011272

2023

中国病毒学
中国科学院武汉病毒研究所,中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子:0.393
ISSN:1674-0769
年,卷(期):2023.(1)
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