首页|Transcriptome profiling highlights regulated biological processes and typeⅢ interferon antiviral responses upon Crimean-Congo hemorrhagic fever virus infection

Transcriptome profiling highlights regulated biological processes and typeⅢ interferon antiviral responses upon Crimean-Congo hemorrhagic fever virus infection

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Crimean-Congo hemorrhagic fever virus(CCHFV)is a biosafety level-4(BSL-4)pathogen that causes Crimean-Congo hemorrhagic fever(CCHF)characterized by hemorrhagic manifestation,multiple organ failure and high mortality rate,posing great threat to public health.Despite the recently increasing research efforts on CCHFV,host cell responses associated with CCHFV infection remain to be further characterized.Here,to better under-stand the cellular response to CCHFV infection,we performed a transcriptomic analysis in human kidney HEK293 cells by high-throughput RNA sequencing(RNA-seq)technology.In total,496 differentially expressed genes(DEGs),including 361 up-regulated and 135 down-regulated genes,were identified in CCHFV-infected cells.These regulated genes were mainly involved in host processes including defense response to virus,response to stress,regulation of viral process,immune response,metabolism,stimulus,apoptosis and protein catabolic pro-cess.Therein,a significant up-regulation of type Ⅲ interferon(IFN)signaling pathway as well as endoplasmic reticulum(ER)stress response was especially remarkable.Subsequently,representative DEGs from these pro-cesses were well validated by RT-qPCR,confirming the RNA-seq results and the typical regulation of IFN re-sponses and ER stress by CCHFV.Furthermore,we demonstrate that not only type Ⅰ but also type Ⅲ IFNs(even at low dosages)have substantial anti-CCHFV activities.Collectively,the data may provide new and comprehensive insights into the virus-host interactions and particularly highlights the potential role of type Ⅲ IFNs in restricting CCHFV,which may help inform further mechanistic delineation of the viral infection and development of anti-CCHFV strategies.

Crimean-Congo hemorrhagic fever virus(CCHFV)Biosafety level-4(BSL-4)pathogenBunyavirusTranscriptomeType Ⅲ interferonER stressVirus-host interactionAntiviral therapeutics

Qiong Mo、Kuan Feng、Shiyu Dai、Qiaoli Wu、Zhong Zhang、Ashaq Ali、Fei Deng、Hualin Wang、Yun-Jia Ning

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State Key Laboratory of Virology and National Virus Resource Center,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071/430207,China

University of Chinese Academy of Sciences,Beijing,101408,China

Centre of Excellence in Science and Applied Technologies,Islamabad,45320,Pakistan

Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071/430207,China

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National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaYouth Innovation Promotion Association of Chinese Academy of SciencesInstitutional Center for Shared Technologies and Facilities of Wuhan Institute of VirologyCAS for technical assistance and the National Virus Resource Center(Wuhan Institute of Virology)for virus resourcesrunning team of National Biosafety Laboratory,Wuhan,Chinese Academy of Sciences

2018YFA0507202321701713187016282161138003

2023

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.(1)
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