首页|Enhanced pathogenicity and transmissibility of H9N2 avian influenza virus in mammals by hemagglutinin mutations combined with PB2-627K

Enhanced pathogenicity and transmissibility of H9N2 avian influenza virus in mammals by hemagglutinin mutations combined with PB2-627K

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H9N2 avian influenza viruses(AIVs)circulate globally in poultry and have become the dominant AIV subtype in China in recent years.Previously,we demonstrated that the H9N2 virus(A/chicken/Eastem China/SDKD1/2015)naturally harbors a mammalian-adaptive molecular factor(627K)in the PB2 protein and is weakly pathogenic in mice.Here,we focused on new markers for virulence in mammals.A mouse-adapted H9N2 virus was serially passaged in mice by infecting their lungs.As expected,infected mice showed clinical symptoms and died at passage six.A comparison between the wild-type and mouse-adapted virus sequences identified amino acid substitutions in the hemagglutinin(HA)protein.H9N2 viruses with the T187P+M227L double mutation exhibited an increased affinity to human-type(SAα2,6Gal)receptors and significantly enhanced viral attachment to mouse lung tissues,which contributed to enhancing viral replication and virulence in mice.Additionally,HA with the T187P+M227L mutation enabled H9N2 viral transmission in guinea pigs via direct contact.AIV pathogenicity in mice is a polygenic trait.Our results demonstrated that these HA mutations might be combined with PB2-627K to significantly increase H9N2 virulence in mice,and this enhanced virulence was achieved in other H9N2 AIVs by generating the same combination of mutations.In summary,our study identified novel key elements in the HA protein that are required for H9N2 pathogenicity in mice and provided valuable insights into pandemic preparedness against emerging H9N2 strains.

H9N2Hemagglutinin(HA)PB2-627KMammalian adaptationPathogenicityTransmissibility

Kaituo Liu、Yaqian Guo、Huafen Zheng、Zhuxing Ji、Miao Cai、Ruyi Gao、Pinghu Zhang、Xiaowen Liu、Xiulong Xu、Xiaoquan Wang、Xiufan Liu

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Joint International Research Laboratory of Agriculture and Agri-Product Safety,The Ministry of Education of China,Yangzhou University,Yangzhou,225009,China

Animal Infectious Disease Laboratory,College of Veterinary Medicine,Yangzhou University,Yangzhou,225009,China

Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis,Yangzhou University,Yangzhou,225009,China

Jiangsu Key Laboratory of Zoonosis,Yangzhou,225009,China

Institute of Translational Medicine,Key Laboratory of Geriatric Disease Prevention and Control of Jiangsu Province,Medical College,Yangzhou University,Yangzhou,225009,China

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National Key Research and Development Project of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaEarmarked Fund for China Agriculture Reasearch SystemPriorty Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

2021YFD1800202317727553207289232072832CARS-40

2023

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.(1)
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