中国病毒学2023，Issue(1) ：66-74.

Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis

Wei Yang Chen Zhang Yan-Hua Wu Li-Bo Liu Zi-Da Zhen Dong-Ying Fan Zheng-Ran Song Jia-Tong Chang Pei-Gang Wang Jing An
中国病毒学2023，Issue(1) ：66-74.
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Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis

Wei Yang 1Chen Zhang 2Yan-Hua Wu 2Li-Bo Liu 2Zi-Da Zhen 2Dong-Ying Fan 2Zheng-Ran Song 2Jia-Tong Chang 2Pei-Gang Wang 2Jing An3
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作者信息

  • 1. Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China;Department of Neurosurgery,Capital Medical University Sanbo Brain Hospital,Beijing,100093,China
  • 2. Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China
  • 3. Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China;Center of Epilepsy,Beijing Institute for Brain Disorders,Beijing,100093,China
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Abstract

Zika virus(ZIKV)poses a serious threat to global public health due to its close relationship with neurological and male reproductive damage.However,deficiency of human testicular samples hinders the in-depth research on ZIKV-induced male reproductive system injury.Organoids are relatively simple in vitro models,which could mimic the pathological changes of corresponding organs.In this study,we constructed a 3D testicular organoid model using primary testicular cells from adult BALB/c mice.Similar to the testis,this organoid system has a blood-testis barrier(BTB)-like structure and could synthesize testosterone.ZIKV tropism of testicular cells and ZIKV-induced pathological changes in testicular organoid was also similar to that in mammalian testis.Therefore,our results provide a simple and reproducible in vitro testicular model for the investigations of ZIKV-induced testicular injury.

Key words

Organoid/3D cell culture/Testicular organoid model/Zika virus(ZIKV)

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基金项目

National Key Research and Development Plan of China(2021YFC2300202)

National Natural Science Foundation of China(U1902210)

National Natural Science Foundation of China(81871641)

National Natural Science Foundation of China(81972979)

National Natural Science Foundation of China(82172266)

National Natural Science Foundation of China(81902048)

Support Project of Highlevel Teachers in Beijing Municipal Universities in the Period of 13th Fiveyear Plan(IDHT20190510)

Beijing Key Laboratory of Emerging Infectious Diseases(DTKF202103)

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子：0.393
ISSN：1674-0769
被引量1
参考文献量62
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