首页|Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis

Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis

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Zika virus(ZIKV)poses a serious threat to global public health due to its close relationship with neurological and male reproductive damage.However,deficiency of human testicular samples hinders the in-depth research on ZIKV-induced male reproductive system injury.Organoids are relatively simple in vitro models,which could mimic the pathological changes of corresponding organs.In this study,we constructed a 3D testicular organoid model using primary testicular cells from adult BALB/c mice.Similar to the testis,this organoid system has a blood-testis barrier(BTB)-like structure and could synthesize testosterone.ZIKV tropism of testicular cells and ZIKV-induced pathological changes in testicular organoid was also similar to that in mammalian testis.Therefore,our results provide a simple and reproducible in vitro testicular model for the investigations of ZIKV-induced testicular injury.

Organoid3D cell cultureTesticular organoid modelZika virus(ZIKV)

Wei Yang、Chen Zhang、Yan-Hua Wu、Li-Bo Liu、Zi-Da Zhen、Dong-Ying Fan、Zheng-Ran Song、Jia-Tong Chang、Pei-Gang Wang、Jing An

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Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China

Department of Neurosurgery,Capital Medical University Sanbo Brain Hospital,Beijing,100093,China

Center of Epilepsy,Beijing Institute for Brain Disorders,Beijing,100093,China

National Key Research and Development Plan of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaSupport Project of Highlevel Teachers in Beijing Municipal Universities in the Period of 13th Fiveyear PlanBeijing Key Laboratory of Emerging Infectious Diseases

2021YFC2300202U190221081871641819729798217226681902048IDHT20190510DTKF202103

2023

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.(1)
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