Abstract
African swine fever(ASF)is etiologically an acute,highly contagious and hemorrhagic disease caused by African swine fever virus(ASFV).Due to its genetic variation and phenotypic diversity,until now,no efficient commercial vaccines or therapeutic options are available.The ASFV genome contains a conserved middle region and two flexible ends that code for five multigene families(MGFs),while the biological functions of the MGFs are not fully characterized.Here,ASFV MGF505-2R-deficient mutant ASFV-Δ2R was constructed based on a highly virulent genotype Ⅱ field isolate ASFV CN/GS/2018 currently circulating in China.Transcriptomic profiling demonstrated that ASFV-Δ2R was capable of inducing a larger number of differentially expressed genes(DEGs)compared with ASFV CN/GS/2018.Hierarchical clustering of up-regulated DEGs revealed that ASFV-Δ2R induced the most dramatic expression of interferon-related genes and inflammatory and innate immune genes,as further validated by RT-qPCR.The GO and KEGG pathway analysis identified significantly enriched pathways involved in pathogen recognition and innate antiviral immunity.Conversely,pharmacological activation of those antiviral immune responses by exogenous cytokines,including type Ⅰ/Ⅱ IFNs,TNF-α and IL-1β,exerted combinatory effects and synergized in antiviral capacity against ASFV replication.Collectively,MGF505-2R is a newly identified inhibitor of innate immunity potentially implicated in immune evasion.
基金项目
National Key R&D Program of China(2021YFD1801300)
Key-Area Research and Development Program of Guangdong Province(2019B020211003)
Chinese Academy of Agricultural Science and Technology Innovation Project(CAAS-ZDRW202006)
Chinese Academy of Agricultural Science and Technology Innovation Project(CAAS-ASTIP-2021-LVRI)
Technology Major Projects of Gansu Province(20ZD7A006)
Technology Major Projects of Gansu Province(NCC0006)
director of Lanzhou Veterinary Research Institute(LVRI-SZJJ-202106)
国家科技期刊平台
NSTL
万方数据