首页|Identification of African swine fever virus MGF505-2R as a potent inhibitor of innate immunity in vitro

Identification of African swine fever virus MGF505-2R as a potent inhibitor of innate immunity in vitro

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African swine fever(ASF)is etiologically an acute,highly contagious and hemorrhagic disease caused by African swine fever virus(ASFV).Due to its genetic variation and phenotypic diversity,until now,no efficient commercial vaccines or therapeutic options are available.The ASFV genome contains a conserved middle region and two flexible ends that code for five multigene families(MGFs),while the biological functions of the MGFs are not fully characterized.Here,ASFV MGF505-2R-deficient mutant ASFV-Δ2R was constructed based on a highly virulent genotype Ⅱ field isolate ASFV CN/GS/2018 currently circulating in China.Transcriptomic profiling demonstrated that ASFV-Δ2R was capable of inducing a larger number of differentially expressed genes(DEGs)compared with ASFV CN/GS/2018.Hierarchical clustering of up-regulated DEGs revealed that ASFV-Δ2R induced the most dramatic expression of interferon-related genes and inflammatory and innate immune genes,as further validated by RT-qPCR.The GO and KEGG pathway analysis identified significantly enriched pathways involved in pathogen recognition and innate antiviral immunity.Conversely,pharmacological activation of those antiviral immune responses by exogenous cytokines,including type Ⅰ/Ⅱ IFNs,TNF-α and IL-1β,exerted combinatory effects and synergized in antiviral capacity against ASFV replication.Collectively,MGF505-2R is a newly identified inhibitor of innate immunity potentially implicated in immune evasion.

African swine fever virus(ASFV)Multigene families(MGFs)MGF505-2RDifferentially expressed genes(DEGs)

Huaguo Huang、Wen Dang、Zhengwang Shi、Mingyang Ding、Fan Xu、Tao Li、Tao Feng、Haixue Zheng、Shuqi Xiao

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College of Veterinary Medicine,Northwest A&F University,Yangling,712100,China

State Key Laboratory of Veterinary Etiological Biology,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,730046,China

College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,730070,China

National Key R&D Program of ChinaKey-Area Research and Development Program of Guangdong ProvinceChinese Academy of Agricultural Science and Technology Innovation ProjectChinese Academy of Agricultural Science and Technology Innovation ProjectTechnology Major Projects of Gansu ProvinceTechnology Major Projects of Gansu Provincedirector of Lanzhou Veterinary Research Institute

2021YFD18013002019B020211003CAAS-ZDRW202006CAAS-ASTIP-2021-LVRI20ZD7A006NCC0006LVRI-SZJJ-202106

2023

中国病毒学
中国科学院武汉病毒研究所,中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子:0.393
ISSN:1674-0769
年,卷(期):2023.(1)
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