首页|GCRV NS38 counteracts SVCV proliferation by intracellular antagonization during co-infection

GCRV NS38 counteracts SVCV proliferation by intracellular antagonization during co-infection

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Viral co-infection has been found in animals;however,the mechanisms of co-infection are unclear.The abun-dance and diversity of viruses in water make fish highly susceptible to co-infection.Here,we reported a co-infection in fish,which resulted in reduced host lethality and illustrated the intracellular molecular mechanism of viral co-infection.The spring viremia of carp virus(SVCV)is a highly lethal virus that infects Cyprinidae,such as zebrafish.The mortality of SVCV infection was significantly reduced when co-infected with the grass carp reovirus(GCRV).The severity of tissue damage and viral proliferation of SVCV was also reduced in co-infection with GCRV.The transcriptome bioinformatics analysis demonstrated that the effect on the host transcripts in response to SVCV infection was significantly reduced in co-infection.After excluding the extracellular interactions of these two viruses,the intracellular mechanisms were studied.We found that the GCRV NS38 remarkably decreased SVCV infection and viral proliferation.The interaction between GCRV NS38 and SVCV nucleoprotein(N)and phosphoprotein(P)proteins was identified,and NS38 downregulated both N and P proteins.Further analysis demonstrated that the N protein was degraded by NS38 indispensable of the autophagy receptor,sequestosome 1(p62).Meanwhile,K63-linked ubiquitination of the P protein was reduced by NS38,leading to ubiquitinated degradation of the P protein.These results reveal that the intracellular viral protein interactions are a crucial mechanism of co-infection and influence the host pathology and expand our understanding in intra-cellular viral interactions co-infection.

Co-infectionSpring viremia of carp virus(SVCV)Grass carp reovirus(GCRV)AntagonizeAutophagyUbiquitination

Zhuo-Cong Li、Long-Feng Lu、Can Zhang、Xue-Li Wang、Jin-Feng Tong、Ke-Jia Han、Dan-Dan Chen、Xi-Yin Li、Li Zhou、Jian-Fang Gui、Shun Li

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Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China

University of Chinese Academy of Sciences,Beijing,100049,China

College of Fisheries and Life Science,Dalian Ocean University,Dalian,116023,China

State Key Laboratory of Freshwater Ecology and Biotechnology,Hubei Hongshan Laboratory,The Innovation Academy of Seed Design,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China

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Strategic Priority Research Program of the Chinese Academy of SciencesNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaYouth Innovation Promotion AssociationKey Program of Frontier Sciences of the Chinese Academy of SciencesChina Agriculture Research System of MOF and MARAAutonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology

XDA240302032018YFD09005042018YFD09002042021YFD120080432073009318730363217302332002431QYZDY-SSW-SMC025CARS-45-07

2023

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.(1)
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