中国病毒学2023，Issue(1) ：142-156.

GCRV NS38 counteracts SVCV proliferation by intracellular antagonization during co-infection

Zhuo-Cong Li Long-Feng Lu Can Zhang Xue-Li Wang Jin-Feng Tong Ke-Jia Han Dan-Dan Chen Xi-Yin Li Li Zhou Jian-Fang Gui Shun Li
中国病毒学2023，Issue(1) ：142-156.
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GCRV NS38 counteracts SVCV proliferation by intracellular antagonization during co-infection

Zhuo-Cong Li 1Long-Feng Lu 1Can Zhang 1Xue-Li Wang 2Jin-Feng Tong 1Ke-Jia Han 2Dan-Dan Chen 1Xi-Yin Li 3Li Zhou 3Jian-Fang Gui 3Shun Li1
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作者信息

  • 1. Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China;University of Chinese Academy of Sciences,Beijing,100049,China
  • 2. University of Chinese Academy of Sciences,Beijing,100049,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian,116023,China
  • 3. University of Chinese Academy of Sciences,Beijing,100049,China;State Key Laboratory of Freshwater Ecology and Biotechnology,Hubei Hongshan Laboratory,The Innovation Academy of Seed Design,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China
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Abstract

Viral co-infection has been found in animals;however,the mechanisms of co-infection are unclear.The abun-dance and diversity of viruses in water make fish highly susceptible to co-infection.Here,we reported a co-infection in fish,which resulted in reduced host lethality and illustrated the intracellular molecular mechanism of viral co-infection.The spring viremia of carp virus(SVCV)is a highly lethal virus that infects Cyprinidae,such as zebrafish.The mortality of SVCV infection was significantly reduced when co-infected with the grass carp reovirus(GCRV).The severity of tissue damage and viral proliferation of SVCV was also reduced in co-infection with GCRV.The transcriptome bioinformatics analysis demonstrated that the effect on the host transcripts in response to SVCV infection was significantly reduced in co-infection.After excluding the extracellular interactions of these two viruses,the intracellular mechanisms were studied.We found that the GCRV NS38 remarkably decreased SVCV infection and viral proliferation.The interaction between GCRV NS38 and SVCV nucleoprotein(N)and phosphoprotein(P)proteins was identified,and NS38 downregulated both N and P proteins.Further analysis demonstrated that the N protein was degraded by NS38 indispensable of the autophagy receptor,sequestosome 1(p62).Meanwhile,K63-linked ubiquitination of the P protein was reduced by NS38,leading to ubiquitinated degradation of the P protein.These results reveal that the intracellular viral protein interactions are a crucial mechanism of co-infection and influence the host pathology and expand our understanding in intra-cellular viral interactions co-infection.

Key words

Co-infection/Spring viremia of carp virus(SVCV)/Grass carp reovirus(GCRV)/Antagonize/Autophagy/Ubiquitination

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基金项目

Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24030203)

National Key Research and Development Program of China(2018YFD0900504)

National Key Research and Development Program of China(2018YFD0900204)

National Key Research and Development Program of China(2021YFD1200804)

National Natural Science Foundation of China(32073009)

National Natural Science Foundation of China(31873036)

National Natural Science Foundation of China(32173023)

National Natural Science Foundation of China(32002431)

Youth Innovation Promotion Association()

Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025)

China Agriculture Research System of MOF and MARA(CARS-45-07)

Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology()

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子：0.393
ISSN：1674-0769
参考文献量47
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