首页|An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

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Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and animals.There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in animals.Rabies virus(RABV)is another important zoonotic pathogen that infects almost all warm-blooded animals and poses a great public health threat.The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and dogs.The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice.Notably,inactivated SRV-nCoV-RBD induced earlier antibody pro-duction than SRV-nCoV-S1,which was maintained at high levels for longer periods.Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broad-spectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against CO VID-19 and rabies in animals.

SARS-CoV-2VaccineRBDChimeric expressionRABV

Haili Zhang、Hongli Jin、Feihu Yan、Yumeng Song、Jiaxin Dai、Cuicui Jiao、Yujie Bai、Jingxuan Sun、Di Liu、Shen Wang、Mengyao Zhang、Jilong Lu、Jingbo Huang、Pei Huang、Yuanyuan Li、Xianzhu Xia、Hualei Wang

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Key Laboratory of Zoonosis Research,Ministry of Education,College of Veterinary Medicine,Jilin University,Changchun,130062,China

Changchun Sino Biotechnology Co.,Ltd.,Changchun,130012,China

Changchun Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Changchun,130122,China

National Key Research and Development Program of ChinaNational Natural Science Foundation of China

2021YFC260020231872487

2023

中国病毒学
中国科学院武汉病毒研究所,中国微生物学会

中国病毒学

CSTPCDCSCD北大核心
影响因子:0.393
ISSN:1674-0769
年,卷(期):2023.(2)
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