Abstract
Norovirus(NoV)is an important cause of viral acute gastroenteritis(AGE).To gain insights into the epidemio-logical characteristics and genetic diversity of NoV among children in Hubei,1216 stool samples from children(≤ 5 years)obtained under AGE surveillance from January 2017 to December 2019 were analyzed.The results showed that NoV was responsible for 14.64％of AGE cases,with the highest detection rate in children aged 7-12 months(19.76％).Statistically significant differences were found between male and female infection rates(x2=8.108,P=0.004).Genetic analysis of RdRp and VP1 sequences showed that NoV GⅡ genotypes were GⅡ.4 Sydney[P31](34.35％),GⅡ.3[P12](25.95％),GⅡ.2[P16](22.90％),GⅡ.4 Sydney[P16](12.98％),GⅡ.17[P17](2.29％),GⅡ.6[P7]and GⅡ.3[P16](each at 0.76％).GⅡ.17[P17]variants were divided into the Kawasaki323-like lineage and the Kawasaki308-like lineage.A unique recombination event was detected between strains of GⅡ.4 Sydney 2012 and GⅡ.4 Sydney 2016.Significantly,all GⅡ.P16 sequences associated with GⅡ.4/GⅡ.2 ob-tained in Hubei were correlated with novel GⅡ.2[P16]variants that re-emerged in Germany in 2016.Antigenic site analysis of complete VP1 sequences from all GⅡ.4 variants from Hubei identified notable variable residues of antibody epitopes.Genotyping under continuous AGE surveillance and observation of the antigenic sites of VP1 are important monitoring strategies for emerging NoV strains.
基金项目
subproject of Major National Science and Technology Projects(2017ZX10103005)
Natural Science Foundation of Hubei Province(2018CFB716)
Natural Science Foundation of Hubei Province(2022CFB015)
Health and Family Planning Scientific Research Project of Hubei Province(WJ2017M137)
Health and Family Planning Scientific Research Project of Hubei Province(WJ2023M107)
NETL
NSTL
万方数据