首页|Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2
Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exten-sively;however,further genetic engineering of HSV-1 is hindered by its complex genome structure.In the present study,we designed and constructed a synthetic platform for HSV-1 based on H129-G4.The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination(TAR)in yeast,and was named H129-Syn-G2.The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus.According to growth curve assay and electron microscopy results,the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus.This synthetic platform will facilitate further manipulation of the HSV-1 genome for the devel-opment of neuronal circuit tracers,oncolytic viruses,and vaccines.
Herpes simplex virus type 1(HSV-1)Neuronal circuit tracersH129-Syn-G2H129-G4Synthetic biology
Han Xiao、Hengrui Hu、Yijia Guo、Jiang Li、Le Wen、Wen-Bo Zeng、Manli Wang、Min-Hua Luo、Zhihong Hu
展开 >
State Key Laboratory of Virology,Center for Biosafety Mega-Science,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China
University of Chinese Academy of Sciences,Beijing,100049,China
NETL
NSTL
万方数据
