中国病毒学2023，Vol.38Issue(3) ：448-458.DOI:10.1016/j.virs.2023.05.006

Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport(ESCRT)protein

Wenyuan Shen Chang Liu Yue Hu Qian Ding Jiabin Feng Zhou Liu Xiaohong Kong
中国病毒学2023，Vol.38Issue(3) ：448-458.DOI:10.1016/j.virs.2023.05.006
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Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport(ESCRT)protein

Wenyuan Shen 1Chang Liu 2Yue Hu 3Qian Ding 2Jiabin Feng 2Zhou Liu 2Xiaohong Kong2
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作者信息

  • 1. Laboratory of Medical Molecular Virology,School of Medicine,Nankai University,Tianjin,300071,China;Department of Spine Surgery,The Second Hospital of Shandong University,Cheeloo College of Medicine,Shandong University,Jinan,250033,China;International Science and Technology Cooperation Base of Spinal Cord Injury,Tianjin Key Laboratory of Spine and Spinal Cord Injury,Tianjin Medical University General Hospital,Tianjin,300052,China
  • 2. Laboratory of Medical Molecular Virology,School of Medicine,Nankai University,Tianjin,300071,China
  • 3. Laboratory of Medical Molecular Virology,School of Medicine,Nankai University,Tianjin,300071,China;Department of Infectious Diseases,Tianjin Second People's Hospital,Tianjin,300192,China
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Abstract

Human immunodeficiency virus-1(HIV-1)encodes simply 15 proteins and thus depends on multiple host cellular factors for virus reproduction.Spastin,a microtubule severing protein,is an identified HIV-1 dependency factor,but the mechanism regulating HIV-1 is unclear.Here,the study showed that knockdown of spastin inhibited the production of the intracellular HIV-1 Gag protein and new virions through enhancing Gag lysosomal degradation.Further investigation showed that increased sodium tolerance 1(IST1),the subunit of endosomal sorting complex required for transport(ESCRT),could interact with the MIT domain of spastin to regulate the intracellular Gag production.In summary,spastin is required for HIV-1 replication,while spastin-IST1 interaction facilitates virus production by regulating HIV-1 Gag intracellular trafficking and degradation.Spastin may serve as new target for HIV-1 prophylactic and therapy.

Key words

HIV-1 replication/Spastin/Gag production/Lysosomal degradation/Endosomal sorting complex required for/transport(ESCRT)

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基金项目

National Natural Science Foundation of China(81571987)

Natural Science Foundation of Tianjin Municipal Science and Technology Commission(20JCQNJC01750)

Natural Science Foundation of Tianjin Municipal Science and Technology Commission(21JCQNJC01600)

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
参考文献量63
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