首页|Elucidating cellular interactome of chikungunya virus identifies host dependency factors

Elucidating cellular interactome of chikungunya virus identifies host dependency factors

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Chikungunya virus(CHIKV)is a re-emerging mosquito-transmitted RNA virus causing joint and muscle pain.To better understand how CHIKV rewires the host cell and usurps host cell functions,we generated a systematic CHIKV-human protein-protein interaction map and revealed several novel connections that will inform further mechanistic studies.One of these novel interactions,between the viral protein E1 and STIP1 homology and U-box containing protein 1(STUB1),was found to mediate ubiquitination of E1 and degrade E1 through the proteasome.Capsid associated with G3BP1,G3BP2 and AAA+ATPase valosin-containing protein(VCP).Furthermore,VCP inhibitors blocked CHIKV infection,suggesting VCP could serve as a therapeutic target.Further work is required to fully understand the functional consequences of these interactions.Given that CHIKV proteins are conserved across alphaviruses,many virus-host protein-protein interactions identified in this study might also exist in other alphaviruses.Construction of interactome of CHIKV provides the basis for further studying the function of alphavirus biology.

Chikungunya virus(CHIKV)InteractomeSTIP1 homology and U-box containing protein 1(STUB1)Valosin-containing protein(VCP)Capsid

Peiqi Yin、Xia Jian、Yihan Liu、Yuwen Liu、Lu Lv、Haoran Cui、Leiliang Zhang

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Department of Clinical Laboratory Medicine,The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital,Jinan,250013,China

NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100176,China

Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China

Medical Science and Technology Innovation Center,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaTaishan Scholars ProgramShandong Provincial Natural Science FoundationAcademic Promotion Programme of ShandongFirst Medical University

8207227082272306tstp20221142ZR2021QC0952019LJ001

2023

10.1016/j.virs.2023.05.007

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.38(4)
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