中国病毒学2023，Vol.38Issue(4) ：497-507.DOI:10.1016/j.virs.2023.05.007

Elucidating cellular interactome of chikungunya virus identifies host dependency factors

Peiqi Yin Xia Jian Yihan Liu Yuwen Liu Lu Lv Haoran Cui Leiliang Zhang
中国病毒学2023，Vol.38Issue(4) ：497-507.DOI:10.1016/j.virs.2023.05.007
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Elucidating cellular interactome of chikungunya virus identifies host dependency factors

Peiqi Yin 1Xia Jian 2Yihan Liu 3Yuwen Liu 4Lu Lv 3Haoran Cui 3Leiliang Zhang5
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作者信息

  • 1. Department of Clinical Laboratory Medicine,The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital,Jinan,250013,China;NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100176,China
  • 2. NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100176,China
  • 3. Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China;Medical Science and Technology Innovation Center,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China
  • 4. Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China
  • 5. Department of Clinical Laboratory Medicine,The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital,Jinan,250013,China;Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China;Medical Science and Technology Innovation Center,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan,250117,China
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Abstract

Chikungunya virus(CHIKV)is a re-emerging mosquito-transmitted RNA virus causing joint and muscle pain.To better understand how CHIKV rewires the host cell and usurps host cell functions,we generated a systematic CHIKV-human protein-protein interaction map and revealed several novel connections that will inform further mechanistic studies.One of these novel interactions,between the viral protein E1 and STIP1 homology and U-box containing protein 1(STUB1),was found to mediate ubiquitination of E1 and degrade E1 through the proteasome.Capsid associated with G3BP1,G3BP2 and AAA+ATPase valosin-containing protein(VCP).Furthermore,VCP inhibitors blocked CHIKV infection,suggesting VCP could serve as a therapeutic target.Further work is required to fully understand the functional consequences of these interactions.Given that CHIKV proteins are conserved across alphaviruses,many virus-host protein-protein interactions identified in this study might also exist in other alphaviruses.Construction of interactome of CHIKV provides the basis for further studying the function of alphavirus biology.

Key words

Chikungunya virus(CHIKV)/Interactome/STIP1 homology and U-box containing protein 1(STUB1)/Valosin-containing protein(VCP)/Capsid

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基金项目

National Natural Science Foundation of China(82072270)

National Natural Science Foundation of China(82272306)

Taishan Scholars Program(tstp20221142)

Shandong Provincial Natural Science Foundation(ZR2021QC095)

Academic Promotion Programme of ShandongFirst Medical University(2019LJ001)

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
参考文献量46
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