首页|Integrated interactome and transcriptome analysis reveals key host factors critical for SARS-CoV-2 infection

Integrated interactome and transcriptome analysis reveals key host factors critical for SARS-CoV-2 infection

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The coronavirus disease 2019(COVID-19)pandemic,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has seriously threatened global public health and caused huge economic losses.Omics studies of SARS-CoV-2 can help understand the interaction between the virus and host,thereby providing a new perspective in guiding the intervention and treatment of the SARS-CoV-2 infection.Since large amount of SARS-CoV-2 omics data have been accumulated in public databases,this study aimed to identify key host factors involved in SARS-CoV-2 infection through systematic integration of transcriptome and interactome data.By manually curating published studies,we obtained a comprehensive SARS-CoV-2-human protein-protein interactions(PPIs)network,comprising 3591 human proteins interacting with 31 SARS-CoV-2 viral proteins.Using the RobustRankAg-gregation method,we identified 123 multiple cell line common genes(CLCGs),of which 115 up-regulated CLCGs showed host enhanced innate immunity and chemotactic response signatures.Combined with network analysis,co-expression and functional enrichment analysis,we discovered four key host factors involved in SARS-CoV-2 infection:IFITM1,SERPINE1,DDX60,and TNFAIP2.Furthermore,SERPINE1 was found to facilitate SARS-CoV-2 replication,and can alleviate the endoplasmic reticulum(ER)stress induced by ORF8 protein through interaction with ORF8.Our findings highlight the importance of systematic integration analysis in understanding SARS-CoV-2-human interactions and provide valuable insights for future research on potential therapeutic targets against SARS-CoV-2 infection.

SARS-CoV-2InteractomeTranscriptomeIntegration analysisER stress

Jie Sheng、Lili Li、Xueying Lv、Meiling Gao、Ziyi Chen、Zhuo Zhou、Jingfeng Wang、Aiping Wu、Taijiao Jiang

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Institute of Systems Medicine,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,100005,China

Suzhou Institute of Systems Medicine,Suzhou,215123,China

Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,School of Basic Medicine,Peking Union Medical College,Beijing,100005,China

Department of Microbiology and Parasitology,College of Basic Medical Sciences,China Medical University,Shenyang 110122,China

Guangzhou Laboratory,Guangzhou,510005,China

State Key Laboratory of Respiratory Disease,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou Medical University,Guangzhou,510120,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaEmergency Key Program of Guangzhou LaboratorySelfsupporting Program of Guangzhou LaboratorySelfsupporting Program of Guangzhou LaboratoryNational Key Research and Development Program of ChinaCAMS Innovation Fund for Medical Sciences(CIFMS)National Key Research and Development Program of China

320706788210237131671371EKPG21-12SRPG22-007SRPG22-0202020YFC08408002021-I2M-1-0612021YFC2302000

2023

中国病毒学
中国科学院武汉病毒研究所,中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子:0.393
ISSN:1674-0769
年,卷(期):2023.38(4)
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