中国病毒学2023，Vol.38Issue(4) ：520-530.DOI:10.1016/j.virs.2023.05.003

SARS-CoV-2 Nsp8 induces mitophagy by damaging mitochondria

Shan Zong Yan Wu Weiling Li Qiang You Qian Peng Chenghai Wang Pin Wan Tao Bai Yanling Ma Binlian Sun Jialu Qiao
中国病毒学2023，Vol.38Issue(4) ：520-530.DOI:10.1016/j.virs.2023.05.003
✕
  • NETL
  • NSTL
  • 万方数据

SARS-CoV-2 Nsp8 induces mitophagy by damaging mitochondria

Shan Zong 1Yan Wu 2Weiling Li 1Qiang You 1Qian Peng 1Chenghai Wang 1Pin Wan 1Tao Bai 3Yanling Ma 4Binlian Sun 1Jialu Qiao5
扫码查看

作者信息

  • 1. Wuhan Institute of Biomedical Sciences,School of Medicine,Jianghan University,Wuhan,430056,China
  • 2. State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China
  • 3. Division of Gastroenterology,Tongji Medical College,Hua Zhong University of Science and Technology,Wuhan,430030,China
  • 4. Department of Respiratory and Critical Care Medicine,Hubei Province Clinical Research Center for Major Respiratory Diseases,NHC Key Laboratory of Pulmonary Diseases,Union Hospital,Tongji Medical College,Hua Zhong University of Science and Technology,Wuhan,430030,China
  • 5. Wuhan Institute of Biomedical Sciences,School of Medicine,Jianghan University,Wuhan,430056,China;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei University of Medicine,Shiyan,442000,China
  • 折叠

Abstract

Autophagy plays an important role in the interaction between viruses and host cells.SARS-CoV-2 infection can disrupt the autophagy process in target cells.However,the precise molecular mechanism is still unknown.In this study,we discovered that the Nsp8 of SARS-CoV-2 could cause an increasing accumulation of autophagosomes by preventing the fusion of autophagosomes and lysosomes.From further investigation,we found that Nsp8 was present on mitochondria and can damage mitochondria to initiate mitophagy.The results of experiments with immunofluorescence revealed that Nsp8 induced incomplete mitophagy.Moreover,both domains of Nsp8 orchestrated their function during Nsp8-induced mitophagy,in which the N-terminal domain colocalized with mitochondria and the C-terminal domain induced auto/mitophagy.This novel finding expands our understanding of the function of Nsp8 in promoting mitochondrial damage and inducing incomplete mitophagy,which helps us to understand the etiology of COVID-19 as well as open up new pathways for creating SARS-CoV-2 treatment methods.

Key words

SARS-CoV-2/Nsp8/Autophagy/Mitophagy/Mitochondrial damage

引用本文复制引用

基金项目

National Natural Science Foundation of China(32100131)

National Natural Science Foundation of China(31670716)

Wuhan Science and Technology Bureau(2020020601012318)

Hubei Key Laboratory of Wudang Local Chinese Medicine Research(Hubei University of Medicine)(WDCM2022008)

Jianghan University(08190006)

Jianghan University(06210035)

Jianghan University(2021yb138)

Jianghan University(2019037)

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
参考文献量41
段落导航相关论文