Abstract
Hantaan virus(HTNV),the prototype virus of hantavirus,could escape innate immunity by restraining type Ⅰ interferon(IFN)responses.It is largely unknown whether there existed other efficient anti-hantaviral tactics in host cells.Here,we demonstrate that the stimulator of interferon genes(STING)strengthens the host IFN-independent anti-hantaviral immunity.HTNV infection activates RIG-I through IRE1-XBP 1-mediated ER stress,which further facilitates the subcellular translocation and activation of STING.During this process,STING triggers cellular autophagy by interacting with Rab7A,thus restricting viral replication.To note,the anti-hantaviral effects of STING are independent of canonical IFN signaling.Additionally,neither application of the pharmacological antagonist nor the agonist targeting STING could improve the outcomes of nude mice post HTNV challenge in vivo.However,the administration of plasmids exogenously expressing the mutant C-terminal tail(ACTT)STING,which would not trigger the type Ⅰ IFN responses,protected the nude mice from lethal HTNV infection.In summary,our research revealed a novel antiviral pathway through the RIG-I-STING-autophagy pathway,which offered novel therapeutic strategies against hantavirus infection.
基金项目
National Natural Science Foundation of China(31970148)
National Natural Science Foundation of China(82172272)
National Natural Science Foundation of China(82202367)
Key Research and Development Program of Shaanxi(2021ZDLSF01-02)
Key Research and Development Program of Shaanxi(2021ZDLSF01-05)
NETL
NSTL
万方数据