首页|miR-204 suppresses porcine reproductive and respiratory syndrome virus(PRRSV)replication via inhibiting LC3B-mediated autophagy
miR-204 suppresses porcine reproductive and respiratory syndrome virus(PRRSV)replication via inhibiting LC3B-mediated autophagy
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Porcine reproductive and respiratory syndrome(PRRS)caused by PRRS virus(PRRSV)has been regarded as a persistent challenge for the swine farms worldwide.microRNAs(miRNAs)play key roles in regulating almost every important biological process,including virus-host interaction.In this study,we found that miR-204 was highly expressed in cells that were not permissive to PRRSV infection compared with cells susceptible to PRRSV infection.Subsequently,we demonstrated that overexpression of miR-204 significantly inhibited PRRSV repli-cation in porcine alveolar macrophages(PAMs).Through bioinformatic analysis,we found that there existed a potential binding site of miR-204 on the 3'UTR of microtubule associated protein 1 light chain 3B(MAP1LC3B,LC3B),a hallmark of autophagy.Applying experiments including luciferase reporter assay and UV cross-linking and immunoprecipitation(CLIP)assay,we demonstrated that miR-204 directly targeted LC3B,thereby down-regulating autophagy.Meanwhile,we investigated the interplay between autophagy and PRRSV replication in PAMs,confirming that PRRSV infection induces autophagy,which in turn facilitates viral replication.Overall,we verify that miR-204 suppresses PRRSV replication via inhibiting LC3B-mediated autophagy in PAMs.These findings will provide a novel potential approach for us to develop antiviral therapeutic agents and controlling measures for future PRRSV outbreaks.
Porcine reproductive and respiratory syndrome virus(PRRSV)miR-204AutophagyLC3B
Yao Yao、Sihan Li、Yingqi Zhu、Yangyang Xu、Siyuan Hao、Shuyuan Guo、Wen-Hai Feng
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State Key Laboratory of Animal Biotech Breeding,Frontiers Science Center for Molecular Design Breeding,Ministry of Agriculture Key Laboratory of Soil Microbiology,and Department of Microbiology and Immunology,College of Biological Sciences,China Agricultural University,Beijing,100193,China
National Natural Science Foundation of China,ChinaNational Major Special Project on New Varieties Cultivation for Transgenic Organisms,China
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