中国病毒学2023，Vol.38Issue(5) ：767-777.DOI:10.1016/j.virs.2023.06.003

Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infection

Jielin Tang Muqing Fu Chonghui Xu Bao Xue Anqi Zhou Sijie Chen He Zhao Yuan Zhou Jizheng Chen Qi Yang Xinwen Chen
中国病毒学2023，Vol.38Issue(5) ：767-777.DOI:10.1016/j.virs.2023.06.003
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Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infection

Jielin Tang 1Muqing Fu 2Chonghui Xu 3Bao Xue 1Anqi Zhou 4Sijie Chen 4He Zhao 3Yuan Zhou 3Jizheng Chen 5Qi Yang 1Xinwen Chen5
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作者信息

  • 1. Guangzhou National Laboratory,Guangzhou,510005,China;State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China
  • 2. Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,510530,China
  • 3. State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China
  • 4. GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou,511436,China
  • 5. Guangzhou National Laboratory,Guangzhou,510005,China;State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China;State Key Laboratory of Respiratory Disease,Guangzhou Medical University,Guangzhou,511436,China
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Abstract

Tick-borne encephalitis virus(TBEV)is an important tick-borne pathogen that poses as a serious public health concern.The coverage and immunogenicity of the currently available vaccines against TBEV are relatively low;therefore,it is crucial to develop novel and effective vaccines against TBEV.The present study describes a novel strategy for the assembly of virus-like particles(VLPs)by co-expressing the structural(core/prM/E)and non-structural(NS2B/NS3Pro)proteins of TBEV.The efficacy of the VLPs was subsequently evaluated in C57BL/6 mice,and the resultant IgG serum could neutralize both Far-Eastern and European subtypes of TBEV.These findings indicated that the VLP-based vaccine elicited the production of cross-subtype reactive antibodies.The VLPs pro-vided protection to mice lacking the type Ⅰ interferon receptor(IFNAR-/-)against lethal TBEV challenge,with undetectable viral load in brain and intestinal tissues.Furthermore,the group that received the VLP vaccine did not exhibit significant pathological changes and the inflammatory factors were significantly suppressed compared to the control group.Immunization with the VLP vaccine induced the production of multiple-cytokine-producing antiviral CD4+T cells in vivo,including TNF-α+,IL-2+,and IFN- γ+T cells.Altogether,the findings suggest that noninfectious VLPs can serve as a potentially safe and effective vaccine candidate against diverse subtypes of TBEV.

Key words

Tick-borne encephalitis virus(TBEV)/Virus-like particle(VLP)/Immunogenicity/Neutralization/Vaccine

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基金项目

National Key Research and Development Program of China(2018YFA0507201)

National Science Foundation of China(32000111)

China Postdoctoral Science Foundation(2020T130021ZX)

China Postdoctoral Science Foundation(2020M672580)

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
参考文献量66
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