首页|Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infection

Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infection

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Tick-borne encephalitis virus(TBEV)is an important tick-borne pathogen that poses as a serious public health concern.The coverage and immunogenicity of the currently available vaccines against TBEV are relatively low;therefore,it is crucial to develop novel and effective vaccines against TBEV.The present study describes a novel strategy for the assembly of virus-like particles(VLPs)by co-expressing the structural(core/prM/E)and non-structural(NS2B/NS3Pro)proteins of TBEV.The efficacy of the VLPs was subsequently evaluated in C57BL/6 mice,and the resultant IgG serum could neutralize both Far-Eastern and European subtypes of TBEV.These findings indicated that the VLP-based vaccine elicited the production of cross-subtype reactive antibodies.The VLPs pro-vided protection to mice lacking the type Ⅰ interferon receptor(IFNAR-/-)against lethal TBEV challenge,with undetectable viral load in brain and intestinal tissues.Furthermore,the group that received the VLP vaccine did not exhibit significant pathological changes and the inflammatory factors were significantly suppressed compared to the control group.Immunization with the VLP vaccine induced the production of multiple-cytokine-producing antiviral CD4+T cells in vivo,including TNF-α+,IL-2+,and IFN- γ+T cells.Altogether,the findings suggest that noninfectious VLPs can serve as a potentially safe and effective vaccine candidate against diverse subtypes of TBEV.

Tick-borne encephalitis virus(TBEV)Virus-like particle(VLP)ImmunogenicityNeutralizationVaccine

Jielin Tang、Muqing Fu、Chonghui Xu、Bao Xue、Anqi Zhou、Sijie Chen、He Zhao、Yuan Zhou、Jizheng Chen、Qi Yang、Xinwen Chen

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Guangzhou National Laboratory,Guangzhou,510005,China

State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China

Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,510530,China

GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou,511436,China

State Key Laboratory of Respiratory Disease,Guangzhou Medical University,Guangzhou,511436,China

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National Key Research and Development Program of ChinaNational Science Foundation of ChinaChina Postdoctoral Science FoundationChina Postdoctoral Science Foundation

2018YFA0507201320001112020T130021ZX2020M672580

2023

10.1016/j.virs.2023.06.003

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.38(5)
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