首页|Identification of a receptor tyrosine kinase inhibitor CP-724714 inhibits SADS-CoV related swine diarrhea coronaviruses infection in vitro

Identification of a receptor tyrosine kinase inhibitor CP-724714 inhibits SADS-CoV related swine diarrhea coronaviruses infection in vitro

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The outbreak of the COVID-19 epidemic in 2020 has caused unprecedented panic among all mankind,pointing the major importance of effective treatment.Since the emergence of the swine acute diarrhea syndrome coronavirus(SADS-CoV)at the end of 2017,multiple reports have indicated that the bat-related SADS-CoV possesses a potential threat for cross-species transmission.Vaccines and antiviral drugs development deserve more attention.In this study,we found that the HER2 phosphorylation inhibitor(CP-724714)inhibited SADS-CoV infection in a dose-dependent manner.Further validation demonstrated that CP-724714 affected at the post-entry stage of SADS-CoV infection cycle.Also,efficient SADS-CoV infection required the activation of HER2 and its cascade Ras-Raf-Mek-Erk signaling pathway.In addition,CP-724714 has a broad-spectrum anti-swine diarrhea coronaviruses activity,and can dose-dependently combat SADS-CoV,porcine epidemic diarrhea virus(PEDV),porcine deltacoronavirus(PDCoV)and transmissible gastroenteritis virus(TGEV)infection in vitro with a specificity index of greater than 21.98,9.38,95.23 and 31.62,respectively.These results highlight the potential utility of CP-724714 or antiviral drugs targeting with HER2 and its cascade Ras-Raf-Mek-Erk signaling pathway as host-targeted SADS-CoV and other related coronaviruses therapeutics.

SADS-CoVCoronavirusAntiviral agentsHER2CP-724714

Ling Zhou、Cheng Li、Ruiyu Zhang、Qianniu Li、Yuan Sun、Yaoyu Feng、Tian Lan、Jingyun Ma

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Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou,510642,China

Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou,510642,China

Guangdong Major Project of Basic and Applied Basic ResearchNatural Science Foundation of Guangdong ProvinceNatural Science Foundation of Guangdong ProvinceScience and Technology Program of Guangzhou City of China

2020B03010300072022A15150124732020A15150102202023B03J1310

2023

10.1016/j.virs.2023.06.010

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.38(5)
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