中国病毒学2023，Vol.38Issue(5) ：801-812.DOI:10.1016/j.virs.2023.07.002

Characterization of bacteriophage BUCT631 lytic for K1 Klebsiella pneumoniae and its therapeutic efficacy in Galleria mellonella larvae

Pengjun Han Mingfang Pu Yahao Li Huahao Fan Yigang Tong
中国病毒学2023，Vol.38Issue(5) ：801-812.DOI:10.1016/j.virs.2023.07.002
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Characterization of bacteriophage BUCT631 lytic for K1 Klebsiella pneumoniae and its therapeutic efficacy in Galleria mellonella larvae

Pengjun Han 1Mingfang Pu 1Yahao Li 2Huahao Fan 1Yigang Tong3
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作者信息

  • 1. College of Life Science and Technology,Beijing University of Chemical Technology,Beijing,100029,China
  • 2. Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology,Beijing,100029,China
  • 3. College of Life Science and Technology,Beijing University of Chemical Technology,Beijing,100029,China;Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology,Beijing,100029,China
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Abstract

Severe infections caused by multidrug-resistant Klebsiella pneumoniae(K.pneumoniae)highlight the need for new therapeutics with activity against this pathogen.Phage therapy is an alternative treatment approach for multidrug-resistant K.pneumoniae infections.Here,we report a novel bacteriophage(phage)BUCT631 that can specifically lyse capsule-type K1 K.pneumoniae.Physiological characterization revealed that phage BUCT631 could rapidly adsorb to the surface of K.pneumoniae and form an obvious halo ring,and it had relatively favorable thermal stability(4-50 ℃)and pH tolerance(pH=4-12).In addition,the optimal multiplicity of infection(MOI)of phage BUCT631 was 0.01,and the burst size was approximately 303 PFU/cell.Genomic analysis showed that phage BUCT631 has double-stranded DNA(total length of 44,812 bp)with a G+C content of 54.1％,and the genome contains 57 open reading frames(ORFs)and no virulence or antibiotic resistance related genes.Based on phylogenetic analysis,phage BUCT631 could be assigned to a new species in the genus Drulisvirus of the subfamily Slopekvirinae.In addition,phage BUCT631 could quickly inhibit the growth of K.pneumoniae within 2 h in vitro and significantly elevated the survival rate of K.pneumoniae infected Galleria mellonella larvae from 10％to 90％in vivo.These studies suggest that phage BUCT631 has promising potential for development as a safe alternative for control and treatment of multidrug-resistant K.pneumoniae infection.

Key words

Bacteriophage BUCT631/Klebsiella pneumoniae/Genomic analysis/Phage therapy/Galleria mellonella

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基金项目

National Key Research and Development Program of China(2018YFA0903000)

National Key Research and Development Program of China(2020YFC2005405)

National Key Research and Development Program of China(2020YFA0712100)

National Key Research and Development Program of China(2020YFC0840805)

Funds for Firstclass Discipline Construction(XK1805)

Funds for Firstclass Discipline Construction(XK1803-06)

Innovation & Transfer Fund of Peking University Third Hospital(BYSYZHKC2022114)

出版年

2023
中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
参考文献量59
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