首页|Genomic similarity and antibody-dependent enhancement of immune serum potentially affect the protective efficacy of commercial MLV vaccines against NADC30-like PRRSV

Genomic similarity and antibody-dependent enhancement of immune serum potentially affect the protective efficacy of commercial MLV vaccines against NADC30-like PRRSV

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Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,com-mercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SX-YL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the ho-mologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain repli-cation in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.

NADC30-like PRRSVModified live virus(MLV)vaccinesGenomic similarityAntibody dependent enhancement(ADE)Cross protection

Yang Li、Lele Xu、Dian Jiao、Zifang Zheng、Zhihao Chen、Yang Jing、Zhiwei Li、Zhiqian Ma、Yingtong Feng、Xuyang Guo、Yumiao Wang、Yuan He、Haixue Zheng、Shuqi Xiao

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State Key Laboratory for Animal Disease Control and Prevention,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,730046,China

College of Veterinary Medicine,Northwest A&F University,Yangling,712100,China

National Natural Science Foundation of ChinaEarmarked Fund for CARS-35,the science and Technology Major Project of Gansu ProvinceScience Foundation for Distinguished Young Scholars of Shaanxi ProvinceNatural Science Foundation of Gansu ProvinceScience and Technology Plan Project of Gansu ProvinceChinese Academy of Agricultural Science and Technology Innovation ProjectStrategic Priority Research Program of the National Center of Technology Innovation for Pigs

3217284622ZD6NA0012021JC-1823JRRA115323JRRA561CAAS-ASTIP-JBGS-20210602NCTIP-XD/C03

2023

10.1016/j.virs.2023.08.010

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.38(5)
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