首页|Reverse genetics systems for SARS-CoV-2:Development and applications

Reverse genetics systems for SARS-CoV-2:Development and applications

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The recent emergence of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)caused serious harm to human health and struck a blow to global economic development.Research on SARS-CoV-2 has greatly benefited from the use of reverse genetics systems,which have been established to artificially manipulate the viral genome,generating recombinant and reporter infectious viruses or biosafety level 2(BSL-2)-adapted non-infectious replicons with desired modifications.These tools have been instrumental in studying the molecular biological characteristics of the virus,investigating antiviral therapeutics,and facilitating the development of attenuated vaccine candidates.Here,we review the construction strategies,development,and applications of reverse ge-netics systems for SARS-CoV-2,which may be applied to other CoVs as well.

SARS-CoV-2Reverse genetics systemsInfectious clonesRepliconsLive attenuated vaccines

Hou-Li Cai、Yao-Wei Huang

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Department of Veterinary Medicine,Zhejiang University,Hangzhou,310058,China

State Key Laboratory for Animal Disease Control and Prevention,South China Agricultural University,Guangzhou,510642,China

Guangdong Laboratory for Lingnan Modern Agriculture,College of Veterinary Medicine,South China Agricultural University,Guangzhou,510642,China

Major Research Plan of the National Natural Science Foundation of ChinaZhejiang Provincial Natural Science Foundation

92269105LZ22C180002

2023

10.1016/j.virs.2023.10.001

中国病毒学
中国科学院武汉病毒研究所，中国微生物学会

中国病毒学

CSTPCDCSCD
影响因子：0.393
ISSN：1674-0769
年,卷(期)：2023.38(6)
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