Objective A method for the determination of oxytetracycline stripping,Determining the stripping time and limitation.Methods According to Chinese Pharmacopoeia 2010 edition two appendix XC second method,Using 0.1mol/L hydrochloric acid solution,100 revolutions per minute,In accordance with the law,45 mi-nutes,According to UV Vis Spectrophotometry.Results the average recovery of oxytetracycline was 96.9%,RSD was 0.12%(n=6).Conclusion the method is simple, rapid,accurate and stable,reproducible.