Apoptosis of pancreatic cancer PANC-1 cells induced by gemcitabine
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目的：研究吉西他滨对人胰腺癌细胞株 PANC-1细胞增殖抑制和诱导凋亡作用机制.方法：MTT 法检测吉西他滨对 PANC-1细胞的增殖抑制作用； Annexin V/ PI 双染法检测细胞凋亡率；Western blot 法检测不同浓度吉西他滨作用后 Smac、Bax 蛋白的表达变化.结果：吉西他滨对 PANC-1细胞生长有明显抑制作用，并呈剂量依赖性；吉西他滨具有诱导人胰腺癌 PANC-1细胞株凋亡作用.Western blot 分析发现吉西他滨能显著上调胰腺癌细胞 PANC-1的 Smac、Bax 蛋白表达水平.结论：吉西他滨可诱导人胰腺癌细胞 PANC-1凋亡，作用与其上调促凋亡因子 Smac、Bax 表达水平有关.
Objective:To study Gemcitabine in vitro inducing human pancreatic cancer cell PANC-1 apoptosis and its molecular mechanism.Methods:Cell proliferation activity was analyzed by MTT colorimetric assay; cell apoptosis rate was observed by PI staining flow cytometry; Smac and Bax protein expression was analyzed by Western blot.Results:MTT colorimetric assay showed:Gemcitabine inhibited pancreatic cancer cell line PANC-1 proliferation with a dose-dependent manner;its value of IC50 5.79ug/ml.Gemcitabine inducing pancreatic cancer cell line PANC-1 apoptosis was confirmed by FCM analysis of PI staining and transmission electron microscopy. Western blot analysis showed that gemcitabine significantly increased Smac,Bax protein expression level in pancreatic cancer cells PANC-1. PANC-1.Conclusion:The apoptosis of human pancreatic cancer PANC-1 cell could be induced by gemcitabine.Gemcitabine pro-apoptotic role may be due to increase of Smac,Bax expression levels.
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