Summary Purpose Investigation brucellosis incidence of human infection, to develop effective measures to prevent the occurrence of brucellosis. Method Acquisition history of close contact with cattle and sheep populations, venous blood 3-5ml enzyme linked immunosorbent assay for serum antibody detection test bases: WS269-2007. Antibody titer test 1:100 as positive clinical fever, joint pain, sweating, fatigue and other symptoms diagnosed as brucellosis.Result A history of close contact with cattle and sheep, a total of 562 people, including Brucella test was positive in 16 the do the antibody titer trial 1:100 of 12 people, 1:200 4 people newly discovered Brucella infection 5cases, combined with the clinical diagnosis of four cases of brucellosis.Conclusion Brucella immune cattle and sheep to carry out, do the work of disinfection, personal protection, susceptible populations vaccinated patients Integrative treatment the outbreaks to do early discovery, early reporting, early isolation and early treatment.