首页|三聚氰胺胶体金免疫层析检测技术的研究

三聚氰胺胶体金免疫层析检测技术的研究

Development of an immunochromatographic assay for detection of Melamine

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目的：建立一种准确、快速、简便的检测三聚氰胺（MEL）的胶体金免疫层析检测技术。方法：采用柠檬酸三钠还原法制备一定粒径的胶体金溶液，并标记抗MEL单克隆抗体，将一定浓度的包被抗原（MEL-OVA）及羊抗鼠二抗IgG喷在硝酸纤维素膜上，制备胶体金免疫层析试纸条。结果：该试纸条检测阈值可达500 ng/mL，检测的准确性为97.2%，试纸条的稳定性和重复性良好。结论：采用该试纸条检测三聚氰胺特异性强，灵敏度高，而且简便快速，无需特殊仪器设备，在液态乳制品质量筛查工作中应用价值较大。
Objective:To develop an accurate, rapid and simple col oidal gold immunochroma-tographic assay (GICA) for the detection of melamine (MEL). Methods:Col oidal gold was prepared through reducing HAuCl4·3H2O by sodium citrate and conjugated with anti-melamine monoclonal antibody. The optimal concentration of MEL-OVA and sheep anti-mouse IgG were coated on nitrocel ulose membrane to get the test strip. Results:The cut-off value was 500 ng/ml and the accuracy was 97.2%.The stabilization and repetition performed wel . Conclusion:The col oid gold strip for detecting melamine is a specific, sensitive and rapid assay for the simul-taneous and qualitative detection of the quality of liquid dairy products.

MelamineMonoclonal antibodyColloid goldTestkit

周磊、钮伟民、何恩奇、朱鹏飞、刘文卫、杨婷、赵春城、赵晓联

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江苏省无锡市疾病预防控制中心 江苏 无锡 214023

江苏省苏微微生物研究有限公司 江苏 无锡 214063

三聚氰胺 单克隆抗体 胶体金 试纸条

XM0906

2013

中国保健营养(中旬刊)
全国卫生产业企业管理协会

中国保健营养(中旬刊)

ISSN：1004-7484
年,卷(期)：2013.(6)
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