Objective:To explore the apply of thinprep cytologic test and living tissue biopsy under colposcope. Methods:Col ected from January 2011 to June 2012 of 7350 cases in our hospital gynecology clinic line TCT examination and selection TCT exception while line 455 colposcopic biopsy patients were retrospectively analyzed. Results:Checked the implementation of TCT 7350 cases found cytological abnormalities 455 cses. IncludeASC-US 219 cases.LSIL 143cases .ASC-H 51 cases .HSIL 39 cases .AIS 1 cases .SCC 2 cases. 216 cases of applying colposcopic biopsy patients. Include normai 192cases CINI 158 cases. CINII 65 cases. CINIII 36 cases SCC 3 cases. Conclusions: Thinprep Cytological Tes is an effective method in screening of cervical diseases. Combining with colposcopic biopsy among patients with abnormal TCT results wil have a good value of apply.